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Haematologica, Vol 90, Issue 7, 988-990
Copyright © 2005 by Ferrata Storti Foundation

Letter

An enzyme-linked immunosorbent assay to screen for inhibitors of the oncogenic anaplastic lymphoma kinase

RH Gunby, CJ Tartari, F Porchia, A Donella-Deana, L Scapozza, and C Gambacorti-Passerini

The discovery of novel anti-cancer drugs targeting anaplastic lymphoma kinase (ALK), an oncogenic tyrosine kinase, raises the need for in vitro assays suitable for screening compounds for ALK inhibition. To this aim we have developed and optimized an ALK-specific enzyme-linked immunosorbent assay that employs a novel ALK peptide substrate and purified ALK kinase domain.


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