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Haematologica, Vol 91, Issue 6, 858-859
Copyright © 2006 by Ferrata Storti Foundation

Letter

Detection and quantification of MBR/JH2 t(14;18) BCL-2 gene rearrangement in follicular lymphoma using a combined real-time polymerase chain reaction assay

E Csernak, E Toth, Z Melegh, T Schneider, A Rosta, and Z Szentirmay

We report our experience with a new real-time polymerase chain reaction (PCR) assay applicable for simultaneous quantification and characterization of MBR/JH translocation in follicular lymphomas. This technique, which combines amplification with the FRET probe with SYBR Green I melting curve analysis, allows efficient detection of tumor cells in bone marrow or peripheral blood and their comparison with the original neoplastic clone.




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Copyright © 2006 by the Ferrata Storti Foundation.