HOME HELP FEEDBACK TABLE OF CONTENTS ARCHIVE SUBSCRIPTIONS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Abdalla, A. O. Articles by Österborg, A. Search for Related Content PubMed PubMed Citation Articles by Abdalla, A. O. Articles by Österborg, A.

Idiotype protein vaccination in combination with adjuvant cytokines in patients with multiple myeloma - evaluation of T-cell responses by different read-out systems

From the Immune and Gene Therapy Laboratory, Cancer Centre Karolinska, Stockholm, Sweden (AOA, LH, IE, HR, HM, AO); Departments of Hematology and Oncology, Karolinska University Hospital Solna (LH, EDR, HM, AO); Department of Oncology/Pathology, Karolinska Institutet, Solna, Stockholm, Sweden (AOA, LH, IE, BN-G, HR, HM, AO)

Correspondence: Håkan Mellstedt, MD, Ph.D., Professor, Department of Oncology, Karolinska University Hospital Solna, SE-171 76, Stockholm, Sweden. E-mail: hakan.mellstedt{at}karolinska.se

Anti-idiotypic T cells were analyzed in myeloma patients (n=18) vaccinated with idiotypic protein together with the adjuvant cytokines granulocyte-macrophage colony-stimulating factor (GM-CSF) and/or interleukin-12 (IL-12). In the group given IL-12/GM-CSF, 78% developed idiotype specific T cells as compared to 22% in the group given only IL-12 (proliferation/ELISPOT assays) (p<0.05). The percentage of immune-responding patients increased when quantitative real time polymerase chain reaction assays for cytokines were included. A predominance of a Th₁ (IFN-/TNF-) immune response was noted in the IL-12 group while a Th₂ (IL-5) response prevailed in the IL-12/GM-CSF group (p = 0.053). Application of multiple read-out systems improved the characterization of the immune response.

Key words: idiotype, vaccination, myeloma, T-cell response.

This article has been cited by other articles:

				A. O. Abdalla, P. Kokhaei, L. Hansson, H. Mellstedt, and A. Osterborg Idiotype vaccination in patients with myeloma reduced circulating myeloma cells (CMC) Ann. Onc., June 1, 2008; 19(6): 1172 - 1179. [Abstract] [Full Text] [PDF]