Haematologica EWOG-MDS website
HOME HELP FEEDBACK TABLE OF CONTENTS ARCHIVE SUBSCRIPTIONS
QUICK SEARCH:   [advanced]


     

Haematologica, Vol 92, Issue 10, 1419-1422
Copyright © 2007 by Ferrata Storti Foundation
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Anderson, P. J.
Right arrow Articles by Sadler, J. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Anderson, P. J.
Right arrow Articles by Sadler, J. E.

Hemostasis

Factor XI/ADAMTS13 complexes are quantitatively insignificant in human plasma

Patricia J. Anderson, David Gailani, Hendrik B. Feys, Weiqiang Gao, Elaine M. Majerus, Karen Vanhoorelbeke, J. Evan Sadler

From the Department of Medicine (PJA, EMM, JES) and Howard Hughes Medical Institute (WG, JES), Washington University School of Medicine, St. Louis, MO 63110 USA; Department of Pathology and Medicine, Vanderbilt University, Nashville, TN 37232 (DG); Laboratory for Thrombosis Research, Interdisciplinary Research Center, Katholieke Universiteit Leuven Campus Kortrijk, B-8500 Kortrijk, Belgium (HBF, KV)

Correspondence: Patricia J. Anderson, Department of Medicine, Washington University School of Medicine, 660 S. Euclid, Box 8066, St. Louis, MO 63110 USA. E-mail: tanderso{at}im.wustl.edu

Reportedly, complexes between factor XI and ADAMTS13 are detected with a commercial ADAMTS13/FXI ELISA kit in plasma and are decreased in thrombotic thrombocytopenic purpura (TTP). Using this kit, control and TTP patient plasma contained varying amounts of signal (25–670% of a reference plasma) but no signal was observed for mixtures of recombinant enzymes, suggesting little interaction. ADAMTS13/FXI complexes were undetectable by immunoprecipitation or gel filtration chromatography in control plasma or mixtures of recombinant proteins. These results suggest that ADAMTS13/FXI complexes are insignificant in plasma and unlikely to affect the function of either protein during normal hemostasis or in TTP.

Key words: von Willebrand Factor, factor XI, ADAMTS13.






HOME HELP FEEDBACK TABLE OF CONTENTS ARCHIVE SUBSCRIPTIONS
Copyright © 2007 by the Ferrata Storti Foundation.