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Human mulipotential mesenchymal/stromal stem cells are derived from a discrete subpopulation of STRO-1^bright/CD34^–/CD45^–/glycophorin-A-bone marrow cells

^* Multiple Myeloma Research Laboratory, Division of Haematology, Institute of Medical and Veterinary Science, Hanson Institute and University of Adelaide, Australia;
^° Mesenchymal Research Laboratory, Division of Haematology, Institute of Medical and Veterinary Science, Hanson Institute and University of Adelaide, Australia;
^# Department of Medicine, University of Melbourne, Melbourne, Australia and Department of Transplantation Immunology, Columbia University, New York, Presbyterian Hospital, New York, USA

Correspondence: Stan Gronthos, Division of Haematology, Institute of Medical and Veterinary Science, Frome Road, Adelaide, 5000, Australia. Phone: international +61.8.82223460. Fax: international +61.8.82223293. E-mail: stan.gronthos{at}imvs.sa.gov.au

Magnetic and flow cytometry-based methods were used to characterize clonogenic stromal cells in human bone marrow. STRO-1^bright stromal cells were found to lack expression of CD34, CD45 and glycophorin-A markers associated with hematopoietic progenitor cells. These studies support the view that these are two distinct stem cell compartments in adult bone marrow.

Key words: MSC, BMSSC, CFU-F, STRO-1, CD34, CD45, glycophorin-A.

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