Haematologica
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Haematologica, Vol 92, Issue 2, 248-251 doi:10.3324/haematol.10746
Copyright © 2007 by Ferrata Storti Foundation
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Stem Cell Transplantation

Lack of evidence of sustained hematopoietic reconstitution after transplantation of unmanipulated adult liver stem cells in monkeys

Michel Drouet, Jean-François Mayol, Françoise Norol, André Peinnequin, Jean-Pierre Zarski, Christian Létoublon, Francis Hérodin

From the Centre de Recherches du Service de Santé des Armées, La Tronche, France (MD, J-FM; AP, FH); Unité de Thérapie cellulaire, Groupe Hospitalier Pitié Salpêtrière Paris, France (FN); Centre Hospitalo-Universitaire Grenoble, France (J-PZ, CL)

Correspondence: Michel Drouet, Experimental Radiohematology Unit, Centre de Recherches du Service de Santé des Armées, 24 Avenue des Maquis du Grésivaudan, 38702, La Tronche, France. E-mail: micheldrouet{at}crssa.net

The aim of this study was to search for hematopoietic potential in the liver of non-human primates. Lethally irradiated (2x5 Gy {gamma}) Cynomolgus macaques were given autologous hepatic mononuclear cells (HMNC) isolated from a liver lobe by perfusion and digestion with 0.1% collagenase. Two monkeys were given intramedullary injections of HMNC (18.6x106/kg, 20.4x106/kg) and two others were co-transplanted with HMNC (14.35x106/kg, 96.5x106/kg) and bone marrow mesenchymal stem cells (0.42x106/kg, 1.16x106/kg). All monkeys exhibited a transient neutrophil recovery from day 22 for 10 days, but failed to produce platelets and remained transfusion-dependent. In conclusion, adult liver stem cells from a monkey model show a low level of in vivo hematopoietic potential, suggesting ex vivo manipulation will be required before clinical use of such cells.

Key words: plasticity, liver, non-human primate, irradiation, mesenchymal stem cells.






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