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Flow cytometry characterization of leukemic phase of nasal NK/T-cell lymphoma in tumor biopsies and peripheral blood

Center for Cell-Based Therapy, Department of Clinical Medicine, School of Medicine-USP, Ribeirão Preto, S.P., Brazil

We report the findings of the immunophenotypic profile of three cases of nasal T/NK cell lymphoma in leukemic phase. Flow cytometry analysis was carried out using cell suspensions of tumor nasal biopsies and peripheral blood. Tumor samples were composed by a mixture of a predominant subset of medium-size true NK cytCD3–, sCD3–, CD56+ cells mixed with a minor subset of medium-size T/NK sCD3+, CD56+ cells. Both subsets were also detected in peripheral blood. In addition, an infiltration of small-size sCD3+, CD56– normal T lymphocytes was also present.