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Platelet aggregation studies: autologous platelet-poor plasma inhibits platelet aggregation when added to platelet-rich plasma to normalize platelet count

From the Unità di Ematologia e Trombosi, Ospedale San Paolo, DMCO Università di Milano, Milan, Italy (MC, MLZ, FL); Dipartimento di Medicina e Specialità Mediche, IRCCS Fondazione Ospedale Maggiore, Mangiagalli e Regina Elena, Università di Milano, Milano, Italy (AL, MLZ)

Correspondence: Marco Cattaneo, MD, Unità di Ematologia e Trombosi, Ospedale San Paolo, Università di Milano, Via di Rusinì 8, 20142 Milano, Italy. E-mail: marco.cattaneo{at}unimi.it

Adjusting platelet count (PC) in platelet-rich plasma (PRP) using platelet-poor plasma (PPP) is recommended for platelet aggregation (PA) studies, but it could also affect PA independently of the decrease in PC. Analysis of aggregation tracings from healthy controls showed that PC correlated with PA in 47 diluted-PRPs, but not in 104 undiluted-PRPs. Dilution of 9 PRPs with PPP progressively decreased PA, while dilution of washed platelets with buffer hardly affected PA. Apyrase partially prevented the inhibitory effect of PPP. Therefore, the practice of diluting PRP with PPP to adjust platelet count should be avoided because it artefactually inhibits PA.

Key words: platelet aggregation, platelet-rich plasma, platelet count.

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