HOME HELP FEEDBACK TABLE OF CONTENTS ARCHIVE SUBSCRIPTIONS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Galmes, A. Articles by Besalduch, J. Search for Related Content PubMed PubMed Citation Articles by Galmes, A. Articles by Besalduch, J.

Long-term hematologic reconstitution and clinical evaluation of autologous peripheral blood stem cell transplantation after cryopreservation of cells with 5% and 10% dimethylsulfoxide at –80°C in a mechanical freezer

From the Hematology Service and Bone Marrow Transplantation Unit (AGa, Agu, AS, MC, MM, MAD, AN, MDB, JM, JBar, JBe); Immunology Service, University Hospital Son Dureta, Palma de Mallorca (JI, NM); Hematology Service, Policlínica Miramar, Palma de Mallorca (AG, AS, JBe); Hematology Service, Hospital Monte Toro, Menorca (PG); Hematology Service, Hospital Can Mises, Ibiza, Balearic Islands, Spain (JBal)

Correspondence: Joan Besalduch, Service of Hematology, University Hospital Son Dureta, Avenue Andrea Doria 55, Palma de Mallorca, 07014, Balearic Islands, Spain. E-mail: jbesalduch{at}hsd.es

We report the long-term evaluation over 12 years of a simplified technique for stem-cell cryopreservation at –80°C without rate-controlled freezing and with 5% (n=251) or 10% (n=47) DMSO as the sole cryoprotectant. Platelet recovery was greater in the 5% DMSO group while long-term hematologic recovery did not differ. Factors influencing a faster hematologic recovery were infusion of more than 2.7x10⁶/kg of CD34⁺ cells, 10% DMSO cryopreservation and G-CSF. We confirm that the procedure is feasible with a reduction in infusion-related toxicity from 60% using 5% DMSO. Differences in hematologic reconstitution were not clinically significant if a minimum of 1.5x10⁶/kg CD34⁺-cells were infused.

Key words: autologous stem-cell transplantation, mechanical freezer, cryopreservation, DMSO.