| HOME | HELP | FEEDBACK | TABLE OF CONTENTS | ARCHIVE | SUBSCRIPTIONS |
|
|
|
||||||
|
||||||||
Malignant Lymphomas |
From the Haematology Departments of Angers (MH, MT-G, NI); Dijon (DC); Nantes (J-LH); Nancy (SB); Reims (CH); Saint Etienne (DG); Brest (CB); and Bobigny (PC); Cytogenetics Department of Angers (LB); Immunology Department of Nancy (M-CB); Haematology Department of Tours (EG) France
Correspondence: Emmanuel Gyan, Service d’hématologie, CHU de Tours Hôpital Bretonneau, 2 boulevard Tonnellé, 37044 Tours CEDEX., E-mail: e.gyan{at}chu-tours.fr
 |
ABSTRACT |
|---|
 TOP ABSTRACT Design and MethodsResultsDiscussionReferences |
|---|
Design and Methods: Patients with favorable characteristics were randomized to receive chemotherapy or ASCT. Patients with unfavorable characteristics (bone marrow involvement and age over 35 years old or leukocytosis >30 x 109/L or failure to achieve medullar complete remission [CR] after one induction course) received a second induction course and ASCT.
Results: Among 45 patients, the CR rate was 71% after induction and 87% after a second induction course. Within the group of 27 patients with favorable characteristics, ten received ASCT and 17 chemotherapy. Ten patients in the group with unfavorable characteristics received ASCT. The 7-year overall survival and progression-free survival rates were 64 and 65%, respectively. Surprisingly, CR obtained after only two induction courses was associated with improved overall survival (p=0.04). None of the known prognostic factors significantly affected survival.
Interpretation and Conclusions: Randomized maintenance or high-dose therapy (HDT) and ASCT or intensified HDT according to initial presentation gave similar overall and relapse-free survival rates. However, HDT allowed sparing of mediastinal irradiation and shortened treatment duration.
Key words: lymphoblastic lymphoma, autologous bone marrow transplantation, lymphoblastic acute leukemia.
Lymphoblastic lymphoma (LBL) and acute lymphoblastic leukemia (ALL) have been grouped together into a single entity in the new WHO classification of hematologic malignancies. Even though the cytological and histological features of LBL and ALL are very similar, these two diseases do differ, mostly by the immunophenotypic origin of the clonal proliferation since 80% of LBL blasts have the T-cell phenotype. 1 Therapeutic studies on T-LBL/ALL are scarce2 and treatments of the type used for non-Hodgkin’s lymphoma (NHL) have given disappointing results.3–5 ALL-type induction regimens have become the standard treatment based on a retrospective analysis of a cohort of 105 children6 and a smaller, prospective trial in adults.7 However, the respective benefit of high-dose therapy (HDT) and autologous stem-cell transplantation (ASCT) or maintenance chemotherapy remains to be evaluated. In addition, the therapeutic value of mediastinal radiotherapy is still being discussed.2,8
The aim of the T-LBL/ALL GOELAL02 trial was to evaluate, after ALL-type induction and consolidation regimens, the outcome of adults with T-LBL/ALL (characterized by a presentation with extramedullary tumor) randomized to receive maintenance chemotherapy combined with prophylactic cranial irradiation after a reinduction course or HDT and ASCT. In addition, LBL patients with leukemic involvement and exhibiting one of the unfavorable characteristics such as age > 35 years, leukocytosis >30 x 109/L or failure to achieve complete remission (CR) in the bone marrow after one induction course received late reinduction and intensified conditioning before ASCT.
|
Design and Methods |
|---|
|
TOPABSTRACTDesign and MethodsResultsDiscussionReferences |
|---|
Diagnosis
The diagnosis was established by histology and immunohistology of lymph-node or tumor biopsy. Cytological examination and flow cytometry immunophenotyping were performed on circulating or bone marrow lymphoblasts and classified according to EGIL criteria after central review. Cytogenetic results were also centrally reviewed.
Treatment
Drugs and their doses used for induction, consolidation, intensification phases of treatment or maintenance are detailed in Table 1. At diagnosis, a first randomization was conducted to assign patients to intravenous or oral steroids (40 mg/m2/day) for induction and served mainly to register every patient. Unpurged stem cells were collected, mainly from bone marrow, 2 weeks after the third course.
|
All patients in group B received the same late reinduction regimen followed by an intensified conditioning regimen with etoposide (40 mg/kg) in addition to cyclophosphamide and TBI with subsequent ASCT or allogeneic bone marrow transplantation (BMT) for patients under 50 years old with an HLA-matched sibling donor. None of the patients received mediastinal irradiation.
Response criteria
Response to treatment was evaluated on day 35 and at the end of the first course of consolidation. CR was defined as complete disappearance of all tumor masses assessed by computed tomography scans. When the mediastinal response was incomplete but at least 70% tumor regression was observed on day 35, a second chemotherapy regimen was initiated according to the protocol. To remain enrolled in the study, all patients had to achieve CR by the end of the second chemotherapy course.
Statistical methods
Sample size
The T-LBL/ALL GOELAL02 trial was part of a larger multicenter GOELAMS study designed to evaluate the impact of early allogeneic BMT or delayed unpurged ASCT for adult ALL10 and the sample size was calculated on this population. Given the rarity of adult T-LBL, we report here the results of our prospective study on LBL patients treated uniformly in the T-LBL/ALL GOELAL02 trial.
Analysis
All primary analyses were conducted using the intention- to-treat rule. We compared the frequencies of risk factors between groups using a 
2 or Fisher’s exact test when necessary. Overall survival (OS) was defined as the time from first randomization to death or date of last follow-up. Relapse-free survival (RFS) was defined as the time from the date of documented CR to the date of events, such as relapse or death from any cause, loss from follow-up or to the date of the last visit.
Survival curves were estimated using the Kaplan- Meier method and differences were analyzed with the log-rank test. All tests were two-sided, with p values <0.05 considered to be statistically significant. The median follow-up time was estimated by reversing the codes for the censoring indicator in a Kaplan-Meier analysis. The last date of follow-up was July 1, 2004.
Logistic regression analysis and Cox proportional hazards regression models were applied, respectively, to select and retain the risk factors significantly affecting the CR rate and time to events. SPSS (Chicago, IL, USA) software version 10.1.3 for Windows was used for these analyses.
|
Results |
|---|
|
TOPABSTRACTDesign and MethodsResultsDiscussionReferences |
|---|
|
3 abnormalities) and four (15%) patients had a T-cell-specific translocation involving the T-cell receptor genes including two cases of t(10;14)(q24;q11), one case of t(8;14)(q24;q11) and one case of t(11;14)(p15;q11). Most of the structural abnormalities were unbalanced and involved chromosomes 7, 9, and/or 11, 1p and 6q. Cytogenetic analyses were performed on a lymph node biopsy for one of the 16 patients without bone marrow involvement and revealed del(6)(q?).
Treatment results
Induction and consolidation
Forty (89%) of 45 eligible patients achieved CR (Figure 1). Thirty-two (71%) patients achieved CR after the first course of induction, one patient died of infection before the day-35 bone marrow evaluation and 12 (27%) patients failed to achieve CR after the first induction course. Two of these 12 patients did not receive the second chemotherapy course: one died in progression, the other was excluded from the protocol and received allogeneic BMT after salvage chemotherapy and is still in CR. CR was obtained after the second course of chemotherapy in eight out of ten patients; treatment was interrupted because of cerebral venous thrombosis related to antithrombin III deficiency in one patient who subsequently died of progressive disease and another patient failed to achieve CR but is alive after receiving an unrelated allogeneic BMT. One patient in CR after induction died of pneumonia. After the second chemotherapy course, 39 (87%) patients were alive in CR and able to receive the planned treatment. As expected, no significant differences were observed in pretreatment characteristics, CR rate or outcome between the patients divided according to the first randomization (intravenous or oral steroids) (data not shown). Thirty LBL patients with or without bone marrow involvement but under 35 years old, with WBC <30 x 109/L and in bone marrow CR after the first chemotherapy course formed group A. Fifteen patients with bone marrow involvement and older than 35 years old (n=5) or with initial WBC 30 x 109/L (n=13) or not in CR after induction (n=3) were included in group B. By definition, all group B patients had BM involvement (as opposed to 47% in group A) and therefore higher WBC counts and lower platelet counts. These patients also had poorer performance status and higher lactate dehydrogenase (LDH) levels and International Prognostic Index (IPI) scores, but the differences were not statistically significant (Table 2).
|
Remission duration and survival
At the time of analysis, the median follow-up was 7.1 years. The median OS for the entire population has not yet been reached with a 7-year OS of 64% (SE 7%) (Figure 2A) and a 7-year RFS of 65% (SE±8%) for the 40 patients in CR after the first two courses (Figure 2B). Eleven (28%) relapsed at a median time of 10 months (range 2–24 months) after achieving CR with the median survival after relapse being 3.1 months (range 0.5–60 months). The median time to relapse was shorter after HDT and ASCT (3.8 months; range 1.9–6.2 months; n=6) than after maintenance therapy (16.4 months; range 1– 22; n=5). Four patients achieved a second CR and three patients survived longer than 1 year after relapse.
|
Group A and B patients had similar response rates. However, patients without bone marrow involvement more often required two chemotherapy courses to achieve CR compared to those with bone marrow involvement with respective CR rates after induction of 56% and 79% contrasting with CR rates after the second chemotherapy course of 94% and 86%, respectively. Survival according to initial presentation and treatment received is presented in Table 3. The 7-year OS of groups A and B did not differ significantly (69% and 60%, respectively) (Figure 2C) with respective 7-year RFS rates of 64% and 67%. The OS and RFS of group A patients, evaluated according to the intent-to-treat rule, were similar after randomization to receive HDT and ASCT (n=10) or reinduction + maintenance therapy (n=17) (Figure 2D). OS and RFS did not vary significantly according to age, WBC, LDH, IPI, stage or level of bone marrow involvement. Pertinently, eight patients who achieved CR only after two induction courses had significantly better OS (p=0.04) than those entering CR after the first induction (Table 3). Among these eight patients who achieved CR after the second course, three did not have mediastinal involvement while five patients with (n=2) or without (n=3) partial bone marrow involvement (< 10%) achieved bone marrow CR after the first course but mediastinal CR only after the second course. The three patients with central nervous system but not bone marrow involvement at diagnosis achieved CR and were randomized to receive consolidation and maintenance chemotherapy; they are still alive in bone marrow CR after the first course of chemotherapy. Two other patients developed central nervous system and hematologic relapses 2 and 8 months after CR, despite our central nervous system prophylaxis regimen.
|
|
Discussion |
|---|
|
TOPABSTRACTDesign and MethodsResultsDiscussionReferences |
|---|
30 x 109/L or not in CR at day 35 received a second chemotherapy course. For these patients, once CR was obtained, an intensified conditioning regimen followed by ASCT was given after additional reinduction. For the 45 patients included in this trial, the 7-year OS and RFS were 64% and 65%, respectively; these results are at least as good as those obtained in the three other main prospective studies on adult LBL in which OS rates were 66%, 51% or 46% in 33, 45 and 119 patients, respectively.7,11,12 However, our population was more homogenous in terms of characteristics and treatment received. Indeed, we included only patients with T-LBL while the European and American trials included 32% and 20%, respectively, of patients with non-T LBL, a disease which clearly evolved differently from T-LBL.1 In addition, their treatment regimens were less homogenous. In the German study, seven patients received the 04/89 trial consolidation while 38 received that of the 05/93 trial.7 Similarly, to increase recruitment, the European trial11 included three groups of 83, 17 and 19 patients who received different induction chemotherapy and conditioning regimens.
The clinical and biological features of LBL in our population, such a predilection for males and a high rate of mediastinal involvement, were similar to those reported previously. However, we included more patients with bone marrow involvement (64%) than in the other main studies, in which 31, 15 and 21% of patients had bone marrow involvement.7,11,12 Different criteria, such as the extent of nodal disease, the degree of bone marrow involvement and the presence of circulating blast cells have been used to define T-LBL and T-ALL. It is now widely accepted that LBL and ALL represent different manifestations of the same underlying disease. In agreement with this concept, we did not observe any survival differences between patients with and without bone marrow involvement.
As previously reported, between 1–2% of patients with T-LBL simultaneously have a myeloproliferative disease with hypereosinophilia.7,12 Our patient with this condition underwent allogeneic BMT and is still in CR of both proliferations.
The GOELAL02 induction,10 derived from the BFM,9 induced a high CR rate (71%) similar to those previously reported3,4,7,13–16 and increased to 89% after the second chemotherapy course. As previously reported,12 patients without bone marrow involvement had a lower CR rate after induction (56%) but achieved similar overall response rates (94%) after the second chemotherapy course. Whether this difference in response rates reflects cell-cycle kinetics should be evaluated in future studies.
Although it has been clearly demonstrated that an ALL-like induction regimen4,7,8,16,17 induces higher CR rates in T-LBL/ALL patients than do conventional or intensive NHL protocols,2–5, 11,14,18–20 the major, unresolved question in the management of T-LBL/ALL remains the consolidation regimen. Indeed, despite obtaining overall CR rates of 80% after administration of an ALL-type regimen in most studies, the less than 50% disease-free survival (DFS) remains disappointing.2 Although allogeneic BMT has been performed in selected LBL patients in CR with DFS around 74% (59–91%),4,11,21 larger registry studies reported 5-year OS rates of 49% and 42% because of high transplant-related mortality.22,23 Therefore, allogeneic BMT was considered in our trial only in relapse or for young patients with refractory disease. The respective contributions of maintenance chemother apy or HDT and ASCT in adult T-LBL/ALL patients in first CR had to be evaluated. HDT performed after a less intensive, NHL-like regimen, including shortterm weekly chemotherapy (MACOP-B, VACOP-B)24 or high-dose NHL-anthracycline-containing regimen5,25 has been disappointing with 3-year, 5-year and 5-year OS of 48%, 32%, and 46%, respectively, suggesting that the quality of the response had to be improved. In the TLBL/ALL-GOELAL02 trial, improved OS and RFS were observed in patients who received HDT and ASCT after ALL-like induction and consolidation regimens or who received maintenance therapy after one course of reinduction. This is in agreement with the results of the European trial.11
Nevertheless, HDT could have several positive effects, as it might help avoid mediastinal irradiation and its long-term sequelae. Despite the absence of mediastinal irradiation, the rate of relapse in the mediastinum in the T-LBL/ALL-GOELAL02 trial was not higher than that in patients who received conventional ALL-like chemotherapy9 followed by prophylactic mediastinal irradiation with 24 Gy (4/40 vs 7/42). These findings are in agreement with results obtained in children, in whom an intensive sequential consolidation regimen led to 7% mediastinal recurrence mostly without mediastinal irradiation6 but contrast with those of the MD Anderson experience of significantly improved freedom from progression after mediastinal radiotherapy in a retrospective non-randomized study.8 HDT also contributes to shortening the duration of chemotherapy for LBL, which can improve the quality of life of these typically young patients, except for young females in whom fertility may be a major issue. Finally, during the 7 years of follow-up of our study, no relapse occurred beyond 1 year post-HDT while some patients given maintenance therapy suffered relapses after 2 years of CR.
As in most of others reports on LBL, classical prognostic factors of ALL or NHL failed to show any prognostic significance in this trial. We did not confirm the poor outcome associated with bone marrow involvement discribed in a recent cohort of 27 patients.26 The observation that a small subset of patients who responded slowly after induction and required a second chemotherapy course to achieve CR had better OS remains to be clarified but is in agreement with the observation by Thomas et al. that a slow response to induction chemotherapy did not have a poor prognistic significance. 12 It is to be hoped that the large trial conducted by the French-Swiss-Belgian Cooperative GRAALL group, currently in progress, will address these points and determine the prognostic value of karyotype, molecular analysis, and early response assessed by 18F-fluorodesoxyglucose positron emission tomography.
|
Acknowledgments |
|---|
|
Footnotes |
|---|
The order of authorship was decided within the GOELAMS group according to each author’s contribution. 
* Mathilde Hunault and Malgorzata Truchan-Graczyk contributed equally to this work.
MH: designed the study, analyzed the data, wrote the paper, included patients in the study; MT-G: designed the sudy, analyzed the data, wrote the paper; DC, J-LH, SB, CH, DG, CB, PC, MD, J-YC, TL, J-PJ, BD, BL, FG, PS-C, BU, AT, MA-V and PB: included patients; LB: performed cytogenetic analysis; M-CB, NI: drafted the study, performed the immunophenotyping analysis, analyzed the data; EG: analyzed the data, revised the manuscript.
The authors reported no potential conflicts of interest
Received for publication October 6, 2006. Accepted for publication September 18, 2007.
|
References |
|---|
|
TOPABSTRACTDesign and MethodsResultsDiscussionReferences |
|---|
This article has been cited by other articles:
|
|
 |
|
  M. Hunault-Berger, P. Chevallier, M. Delain, C.-E. Bulabois, S. Bologna, M. Bernard, I. Lafon, J. Cornillon, A. Maakaroun, A. Tizon, et al. Changes in antithrombin and fibrinogen levels during induction chemotherapy with L-asparaginase in adult patients with acute lymphoblastic leukemia or lymphoblastic lymphoma. Use of supportive coagulation therapy and clinical outcome: the CAPELAL study Haematologica, October 1, 2008; 93(10): 1488 - 1494. [Abstract] [Full Text] [PDF]
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| HOME | HELP | FEEDBACK | TABLE OF CONTENTS | ARCHIVE | SUBSCRIPTIONS |
