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JAK2V^617F detection and dosage of serum erythropoietin: first steps of the diagnostic work-up for patients consulting for elevated hematocrit

François Girodon^*,, Eric Lippert^°, Pascal Mossuz^#, Irène Dobo^@, Nathalie Boiret-Dupré, Jean-François Lesesve, Sylvie Hermouet^**, Vincent Praloran^°

^* Laboratoire d’Hématologie, CHU de Dijon, Dijon, France
^° Laboratoire d’Hématologie, CHU de Bordeaux, Bordeaux, France
^# Laboratoire d’Hématologie, CHU de Grenoble, Grenoble, France
^@ Laboratoire d’Hématologie, CHU d’Angers, Angers, France
Laboratoire d’Hématologie, CHU de Clermont-Ferrand, Clermont-Ferrand, France
Laboratoire d’Hématologie, CHU de Nancy, Nancy, France
^** Laboratoire d’Hématologie, CHU de Nantes, Nantes, France

Correspondence: François Girodon, Laboratoire d’Hématologie, Hôpital du Bocage, CHU de Dijon, Dijon, France. Phone: international +33.380293047. Fax: international +33.380293660. E-mail: francois.girodon{at}chu-dijon.fr

	ABSTRACT

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The predictive values of common biological criteria for the diagnosis of polycythemia vera were studied in a cohort of patients with high hematocrit. We found JAK2V^617Fand erythropoietin assays were the most relevant first tests. Classification of patients according to their JAK2V^617F status and erythropoietin levels facilitated the choice of further diagnostic investigations.

Key words: erythrocytosis, polycythemia vera, JAK2V^617F, erythropoietin, myeloproliferative disorders.

The discovery that more than 90% of people with polycythemia vera (PV) have the V617F mutation of JAK2 (JAK2V^617F )^1–5 calls for a re-assessment of the usefulness of Polycythemia Vera Study Group (PVSG) or World Health Organization (WHO) criteria for diagnosing PV. New guidelines have recently been proposed; however, they were not based on biological and clinical data from large series of untreated patients.^6–8 In the present study, in which the clinical and biological data of 419 untreated patients (168 with known JAK2 status) consulting for an elevated hematocrit were collected, we compared the PVSG, WHO and new PV criteria and tried to determine the most efficient diagnostic strategy. Males with a hematocrit (Hct) >50% and females with a Hct >48% were included after informed consent, following the guidelines of the ethical committee of "Région Bourgogne". The patients were evaluated before treatment, except for a small number who needed an emergency phlebotomy. The 57 patients with a Hct >60% (males) or >56% (females) were considered as having true erythrocytosis and red cell mass (RCM) was not measured. Standardized endogenous erythroid colony (EEC) assays, dosage of serum erythropoietin and detection of JAK2V^617F by quantitative polymerase chain reaction in blood granulocytes were performed as described elsewhere.^5,9

The PVSG criteria were more stringent than the WHO criteria since 39 patients diagnosed as having PV by the WHO criteria were considered to have idiopathic erythrocytosis (IE) according to the PVSG. JAK2V^617F was present in 13/13 WHO-positive/PVSG-negative PV patients who were tested. The WHO criteria were then used as a reference to evaluate the value of other PV markers in 168 patients for whom JAK2 status could be assessed. The positive and negative predictive values (PPV, NPV) of JAK2V^617F with the WHO criteria as the reference were 92% and 97%, respectively. We used an alternate classification to evaluate the PPV and NPV of EEC, serum erythropoietin level and bone marrow histology (BMH). This classification was based on the WHO criteria minus the studied parameter which was replaced by JAK2V^617F, considered as a major criterion. This modified classification allowed us to determine the PPV and NPV of EEC (95% and 83%, respectively), low (<3.3 IU/L) serum erythropoietin (86% and 89%, respectively) and bone marrow histology (91% and 77%, respectively). Although JAK2 status had excellent PPV and NPV, used singly none of the studied parameters was sufficient to establish or refute the diagnosis of PV. Among the 71 patients with the JAK2 mutation, six did not fulfil the WHO criteria (Table 1). Four had low erythropoietin levels: patients A86 and A98, initially classified as having apparent erythrocytosis eventually developed a complete phenotype of PV with a RCM >1.25 and EEC formation. For patients N89 and B164 (who had only low serum erythropoietin and were thus classified as having idiopathic erythrocytosis, a very high hematocrit, the presence of JAK2V^617F and the absence of a cause of secondary erythrocytosis led to the diagnosis of PV. Two other JAK2V^617F -positive patients had normal erythropoietin levels. They do not have any WHO criteria of PV and show no signs of evolution in the absence of treatment 2 years after diagnosis. For these reasons, despite their JAK2V^617F positivity, these patients were considered to have idiopathic erythrocytosis. Ninety-seven patients did not have the JAK2 mutation. Three of them fulfilled the WHO criteria of PV, with EEC. One had low erythropoetin levels and two had normal levels, but the assays were done after phlebotomy.

We then asked whether a combination of tests could reach a PPV and/or NPV of 100%. This was possible only by combining JAK2 status, erythropoietin levels and EEC status and retaining the diagnosis when two of these three criteria were in favor of PV. Since EEC is not routinely available in every hospital, we evaluated the interest of first subgrouping patients according to their JAK2 status and erythropoietin levels to guide further explorations (Figure 1). This strategy allowed a diagnosis of PV to be affirmed in 61/72 patients finally classified as having PV (group 1) and definitively excluded PV for 11 patients with erythropoietin >17.7 IU/L (group 5).⁹ For patients in other groups, a confirmatory test (mainly EEC) should be performed if JAK2V^617F is detected or if serum erythropoietin concentration is low (groups 2 and 3). In groups 4 and 5 (normal/high erythropoietin), RCM or a careful search for a cause of secondary erythrocytosis should be the first steps. This strategy prioritizes the most relevant investigations, thus saving time and money.

View larger version (30K): [in this window] [in a new window] [Download PPT slide]   Figure 1. Diagnostic work-up of erythrocytoses with JAK2V617F detection and serum erythropoietin (Epo) dosage as the first steps. Patients were classified in groups 1 (JAK2V617F -positive, low serum Epo), 2 (JAK2V617F -positive, normal/high Epo), 3 ( JAK2V617F-negative, low serum Epo), 4 (JAK2V617F -negative, normal serum Epo) and 5 (JAK2V617F-negative, high serum Epo). For each group, the most efficient exploration strategy is indicated. Hct: hematocrit; EEC: endogenous erythroid colony; RCM: red cell mass; PV: polycythemia vera; AE: apparent erythrocytosis; IE: idiopathic erythrocytosis; SE: secondary erythrocytosis.

	Acknowledgments

 
we thank Dr. Céline Schaeffer (CHU Dijon) and Dr. Magali Donnard (CHU Limoges) for collecting patients’ data, Dr. Malik Fardeheb and Dr. Christine Binquet (Centre d’Investigation Clinique - Epidémiologie Clinique/Essais Cliniques, Univ de Bourgogne, Dijon, France) for their help in analyzing the data. We are indebted to colleagues from the Departments of Clinical Haematology of the Centres Hospitaliers of Angers, Bayonne, Bordeaux, Châlon-sur-Saône, Clermont-Ferrand, Dax, Dijon, Laval, Libourne, Limoges, Lorient, Nancy, Nantes, La Roche-sur-Yon, and Saumur for providing samples.

	Footnotes

 
FG and EL contributed equally to this work

Funding: this work was supported by grants from the Programme Hospitalier de Recherche Clinique of the Région Bourgogne and the Grand Ouest and Aquitaine committees of the Ligue Nationale contre le Cancer.

	References

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