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Red Cell Disorders |
Department of Medicine (Thrombosis Research) (PG, SY JAL, PT) and Pathology Baylor College of Medicine (SKD, AL, PT) and Michael E. DeBakey Veterans Affairs Medical Center Houston, Tx, USA (PT)
Correspondence: Perumal Thiagarajan, MD, Michael E. DeBakey VA Medical Center, Mail stop #113, 2002 Holcombe Blvd, Houston, TX 77030, USA. Phone: international +1.713.7947873. Fax: international +1.713.7947657. E-mail.perumalt{at}bcm.tmc.edu
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ABSTRACT |
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Vß3. These results indicate that lactadherin can mediate phosphatidyl-serine-expressing sickle cell adhesion to the endothelium.
Key words: lactadherin, sickle cell anemia, phosphatidyl-serine, endothelial adhesion.
In sickle cell anemia, there is a strong correlation between the red cell adhesion to endothelium and clinical severity.1 Increased exposure of sickle red blood cells to phosphatidylserine is one the factors that promote adhesion2. Lactadherin (also known as milk fat globule-EGF factor 8), is a phosphatidylserine-binding glycoprotein containing an EGF-like domain at the amino terminus with the RGD sequence and two C-domains at the carboxy terminus. It promotes engulfment of phosphatidylserine-containing apoptotic cells3 and sickle red blood cells4 by macrophages. We have examined its role in sickle cell adhesion to the endothelium.
Bovine Lactadherin was isolated as described before.4,5 The monoclonal antibody L688 (IgA
) was derived from a mouse immunized with bovine lactadherin.6 The splenocytes were harvested and fused to non-secreting myeloma cell line NS-1. The resulting hybrids were screened for antibody production against human recombinant lactadherin by ELISA. One hybrid that showed significant binding was expanded, retested, and cloned by limiting dilution. The antibody was purified from the tissue culture supernatant by ammonium sulfate precipitation and gel filtration on Sephacryl S 200 (Amersham-Pharmacia, Piscataway, NJ, USA).
After receiving their written informed consent, blood was collected from volunteers or patients with sickle cell anemia (homozygous SS). The study was approved by the committee for the protection of human subjects at the Baylor College of Medicine. None of the patients were clinically in a pain crisis. Washed red blood cells were incubated with N-ethylmaleimide (10 mM) and ionophore A23187 (4 µM) to induce transbilayer movement of phosphatidylserine as described by Kuypers et al.2 A parallel-plate flow chamber was assembled with the histamine-treated endothelial cells forming the bottom of the flow chamber and mounted onto an invertedstage microscope equipped with a high-speed digital camera as previously described.7 Washed red cells, resuspended at 1% hematocrit in autologous plasma or in serum-free medium 199, containing 1% bovine serum albumin, were perfused through the chamber to generate a wall shear stress of 1.0 dyne/cm2 for five minutes and the images of adherent red cells were acquired. These images were analyzed offline using MetaMorph software (Universal Images, West Chester, PA) in 15 random fields at 40 xmagnification. The adhesion is expressed as the number of red blood cells in a field.
All experimental values are represented as mean ± standard error. Analysis of the difference was performed using Student’s t-test for paired and unpaired data. Values were regarded significant at p<0.05.
Sickle red blood cells, in autologous platelet-free plasma, adhered to histamine-treated human umbilical vein endothelial cells avidly at the venular shear stress of 1.0 dyne/cm2 in a parallel-plate flow chamber as described.1 L688 significantly inhibited the adhesion compared to an isotype matched control monoclonal antibody MOPC-320 (Figure 1A). In five separate experiments involving five patients, L688 inhibited the adhesion by 24–30% (p<0.001). These results show that lactadherin is one of the molecules that mediate adhesion of sickle red blood cells to the endothelium under flow in a whole blood milieu. In a separate set of experiments, we examined the adhesion in a serum-free tissue culture medium. Lactadherin stimulated sickle red blood cell adhesion to endothelial cells in a concentration-dependent manner (Figure 1B).
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Vß3 (Figure 2A). Furthermore, phosphatidylserine vesicles also inhibited sickle cell adhesion. These results show that lactadherin bridges the phosphatidylserine-expressing sickle red blood cells and endothelial cells by binding to phosphatidylserine on red blood cell surface and integrin V'3 on the endothelial cell surface.
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The data presented here show that lactadherin, which is present in plasma,10 is a mediator of phosphatidyl-serine-expressing sickle cell adhesion to the endothelium under physiological venular shear stress via Vß3. Lactadherin also promotes the phagocytosis of phosphatidylserine-expressing sickle red blood cells by macrophages.4 We suggest that lactadherin-bound sickle red blood cells that escape ingestion by macrophages are more adhesive towards endothelium. In conclusion, lactadherin appears to be involved both in sickle cell clearance from the circulation and in adhesion to the endothelium.
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Footnotes |
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Funding: supported by a grant from the Veterans Affairs Research Service.
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TOPABSTRACTReferences |
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This article has been cited by other articles:
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  S. K. Dasgupta, H. Abdel-Monem, P. Niravath, A. Le, R. V. Bellera, K. Langlois, S. Nagata, R. E. Rumbaut, and P. Thiagarajan Lactadherin and clearance of platelet-derived microvesicles Blood, February 5, 2009; 113(6): 1332 - 1339. [Abstract] [Full Text] [PDF]
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