Haematologica, Vol 93, Issue 5, 646-648 doi:10.3324/haematol.13194
Copyright © 2008 by Ferrata Storti Foundation
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Editorials and Perspectives

Molecular basis of thrombocytosis

Mario Cazzola

Department of Hematology, University of Pavia Medical School and Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy. E-mail: mario.cazzola{at}unipv.it

The bone marrow of a normal individual produces 1x1011 platelets per day,1 i.e., about half of the number of red cells (2x1011 per day). Platelets derive from fragmentation of megakaryocytes through a biogenetic process that involves long cytoplasmic extensions called proplatelets.2 Megakaryocytes develop from committed progenitors referred to as colony-forming unit megakaryocyte (CFU-MK), a heterogeneous population of cells that are capable of proliferation.3 At a certain stage, CFU-MK stop proliferating and enter endomitosis, thus becoming megakaryoblasts or immature megakaryocytes. The process of endomitosis involves DNA replication without cellular division, and gives rise to a polyploid cell with a single polylobulated nucleus, the typical pattern of a mature megakaryocyte.4

Megakaryocytopoiesis and platelet production are mainly regulated by thrombopoietin although cytokines such interleukin-11 and interleukin-6 may also play a role.5 In particular, these latter cytokines would allow the progenitors to relocate to a microenvironment that is permissive and instructive for megakaryocyte maturation and platelet production.6

The effect of thrombopoietin on megakaryocytopoiesis is mediated through its receptor, c-Mpl (CD110),7 which is found on both megakaryocytes and platelets, although at low levels on these latter. Since thrombopoietin is produced at a constant rate by the liver, its concentration is regulated – at least in part – by binding to its receptor on circulating platelets. When the platelet count decreases (e.g., as a result of antibody-mediated destruction), increased plasma levels of thrombopoietin expand megakaryocytopoiesis and platelet production. Conversely, when the platelet count rises, more thrombopoietin binds to platelet c-Mpl receptors and less ligand is available for megakaryocyte receptors, leading to slowed megakaryocytopoiesis. A number of clinical observations, however, suggest that other regulatory mechanisms may operate. In an elegant study,8 Skoda and co-workers showed that the translation of thrombopoietin mRNA is physiologically almost completely inhibited by the presence of uAUG codons in the 5'-untranslated region. They also found that a splice variant was more efficiently translated than the two regularly spliced isoforms, suggesting that regulation of alternative splicing may serve as an additional control mechanism for thrombopoietin production.

The platelet count may range from 100x109/L up to 400x109/L in healthy individuals, and only occasional apparently normal subjects show values between 400 and 450x109/L. A platelet threshold count of 450x109/L appears useful for making a diagnosis of thrombocytosis in clinical practice, while values between 350 and 450x109/L require follow-up with sequential evaluations.

Thrombocytosis can be classified into three major categories as shown in Table 1: i) hereditary or familial thrombocytosis; ii) thrombocytosis associated with myeloproliferative and/or myelodysplastic disorders (clonal thrombocytosis), and iii) reactive (secondary) thrombocytosis.9


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  		Table 1. Conditions associated with thrombocytosis.

Most patients with thrombocytosis have reactive thrombocytosis. Indeed, in a retrospective German study,30 643/732 (88%) patients with a platelet count of more than 500x109/L were found to have secondary thrombocytosis, mainly related to inflammatory conditions. Primary thrombocytosis is mainly found in myeloproliferative disorders, and particularly in essential thrombocythemia (Table 1). Somatic mutations of JAK2 or MPL lead to more efficient translation of the thrombopoietin signal and platelet production.

Interestingly, an activating germline mutation of MPL has been found in families with essential thrombocythemia.15,16 This mutation [MPL (S505N)] has also been found as an acquired somatic mutation in patients with essential thrombocythemia or primary myelofibrosis.21 This reinforces the concept that this is indeed a causative mutation responsible for dysregulated platelet production, irrespective of its congenital or acquired nature.

In other families with a similar condition, however, the molecular mechanism is a gain-of-function mutation in the thrombopoietin gene (THPO) that leads to increased thrombopoietin production.10–13 The THPO mutations identified so far alter the 5’-untranslated region of the THPO mRNA, which contains upstream open reading frames (uORF) that inhibit mRNA translation. All mutations remove the inhibitory uORF and lead to increased translation of the THPO mRNA, causing overproduction of thrombopoietin and platelets. This represents a remarkable example of a novel molecular mechanism of disease that we defined "translational pathophysiology".31

In this issue, Liu and co-workers14 describe studies on a large Polish family with hereditary thrombocythemia associated with a de novo splice donor mutation in THPO involving increased thrombopoietin production. Interestingly, megakaryocyte morphology in these patients differs from that in patients with sporadic essential thrombocythemia associated with JAK2 or MPL mutations. These patients have symptoms of impaired microcirculation and require aspirin therapy, but do not require cytoreductive therapy for prevention of thrombosis. Likely, they lack the increased platelet and leukocyte activation which is found in essential thrombocythemia associated with JAK2 (V617F).32 Indeed, activating THPO mutations have not been detected in patients with sporadic thrombocythemia,33 and are, therefore, unlikely to be causative somatic mutations.

As indicated by Skoda and Prchal,34 studies of families with myeloproliferative disorders can contribute greatly to our understanding of the molecular basis of sporadic conditions. However, we must clearly distinguish between familial disorders associated with germline mutations (as those reported in Table 1) and familial myeloproliferative disorders associated with somatic mutations of JAK2 or MPL.35 What is inherited in these latter is not the JAK2 or MPL mutation, but rather a genetic predisposition to its acquisition, as remarkably indicated by the two families described by Pietra and co-workers.36

References

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