Published online 19 February 2009
Haematologica, Vol 94, Issue 4, 596-598 doi:10.3324/haematol.2008.000752
Copyright © 2009 by Ferrata Storti Foundation
Lisa M. Rimsza1 ,
Wing C. Chan2 ,
Randy D. Gascoyne3 ,
Elias Campo4 ,
Elaine S. Jaffe5 ,
Louis M. Staudt5 ,
Jan Delabie6 ,
Andreas Rosenwald7 ,
Shawn P. Murphy8
In RFX5, 3 potentially significant mutations included a G to C mutation at +4 in intron 5 (sample 435), P409R in 2 samples (samples S8, D8), and an R470stop mutation identified in a PTCL MHCII-sample (sample S8) that encoded a truncation mutant. Other mutations were considered non-significant. 
Funding: grant from the American Cancer Society, #RSG0605501L1B. The authors have no relevant conflicts of interest to disclose.
Haematologica, Vol 94, Issue 4, 596-598 doi:10.3324/haematol.2008.000752
Copyright © 2009 by Ferrata Storti Foundation
Malignant Lymphomas |
CIITA or RFX coding region loss of function mutations occur rarely in diffuse large B-cell lymphoma cases and cell lines with low levels of major histocompatibility complex class II expression
1 Department of Pathology, University of Arizona, Tucson, Arizona, USA
2 Dept. of Pathology, University of Nebraska, Omaha, Nebraska, USA
3 Department of Pathology, British Columbia Cancer Agency, Vancouver, British Columbia, Canada
4 Department of Pathology, University of Barcelona, Barcelona, Spain
5 Metabolism Branch, National Cancer Institute, Bethesda, Maryland, USA
6 Department of Pathology, Radium Hospital, Oslo, Norway
7 Department of Pathology, University of Würzburg, Würzburg, Germany
8 Departments of Obstetrics and Gynecology, Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA
Correspondence: Lisa Rimsza, MD, Department of Pathology 1501 N. Campbell Ave, Box 245043, Tucson, AZ 85724-5043 USA. Phone: international +520.6268396. Fax: international +520-626-6081. E-mail:lrimsza{at}email.arizona.edu
Loss of major histocompatibility complex class II (MHCII) gene expression was associated with poor outcome in diffuse large B-cell lymphoma (DLBCL) in several studies.1–3 The mechanism for lost expression is unknown. MHCII gene expression is controlled by several transcription factors, including RFX (composed of RFXB, RFX5, and RFXAP), CREB, and NF-Y. These transcription factors interact with a master transactivator protein class II transactivator (CIITA) to form an enhanceosome complex.4 In the Bare Lymphocyte Syndrome (BLS), MHCII gene expression is absent due to small deletions or point mutations in the coding or splicing regions of either CIITA or an RFX subunit.5 This study investigated whether DLBCL with low MHCII expression had mutations similar to BLS.
DNA from 46 patient tumors, for which gene expression profiling was available, was obtained from the Lymphoma and Leukemia Molecular Profiling Project (LLMPP).1 These cases had been previously reviewed by a panel of expert hematopathologists and each contained a minimum of 70% tumor. Twenty-three were from de novo DLBCL in the lowest 10% of MHCII expression, 4 were in the 10–25% range, and 4 were transformed or relapsed DLBCL in the lowest 10%. Fifteen LLMPP cases were de novo MHCII+ samples in the upper 25–100% of expression. Fourteen additional samples were sequenced, including 1 MHCII+ DLBCL, 1 MHCII-DLBCL, and 2 MHCII-peripheral T-cell lymphomas (PTCL), unspecified. MHCII status for these samples was determined by immunohistochemistry. Seven reactive lymph nodes and 3 tonsils were considered positive controls. Ten cell lines (7 MHCII+, 3 MHCII–) were also sequenced.
Six PCR reactions using the Multiplex PCR kit (Qiagen, Valencia, CA, USA) analyzed the coding regions of all 4 genes (primer sequences and conditions available on request) using the Multiplex PCR kit (Qiagen). Amplified DNA was purified with the Qiaquick PCR Purification Kit (Qiagen) and sequenced. Possible mutations were confirmed by sequencing the opposite strand. Gap software was used to compare sequences to NCBI genomic sequences. In 3 samples (129, 172, 435), 2 divergent overlapping sequences appeared. For these, the relevant amplicon from a separate, non-multiplex PCR was cloned into the pGEM-T Easy vector (Promega, Madison, WI) and sequenced. Significant mutations were those resulting in a non-conserved change in amino acid sequence or change in a splice site and were not seen as the only allele in a known MHCII+ sample. Mutations were considered non-significant if they were silent mutations that caused no change in amino acid sequence, conserved mutations that caused no change in class of amino acid, or exclusive mutations in a sample known to express MHCII. Results summarized in Table 1. Only 3 mutations were considered unequivocal causes of loss of MHCII expression, by virtue of being present exclusively in MHCII-samples and by encoding mutations which would exclude required known functional regions of the coded proteins. In one case (in MHCII-DLBCL sample 8), an exclusive insertional mutation was identified in CIITA exon 11, 5’ of the codon for aa 810. This insertion would result in a truncated CIITA protein lacking C-terminal sequences necessary for function, including a nuclear localization sequence and the leucine rich repeat (LRR) regions.6,7 An exclusive Q252stop mutation was detected in exon 3 of RFXAP in one of the MHCII- PTCL samples (sample S8), which created a termination codon that would truncate RFXAP at aa252. This mutation would clearly disrupt RFXAP function, for the sequences of RFXAP C-terminal of aa 252 are required for productive MHCII transcription.8 A large deletion in exons 11 and 12 of CIITA was observed in the RJ2.2.5 line, a 
radiation induced MHCII-derivative of Raji that expresses a CIITA mRNA containing an 1811 base deletion at bp1122-2933. This deletion produced a truncated protein of 353 aa because of a frameshift at aa335 and a translational stop codon, creating a non-functional CIITA protein.9
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Table 1. Mutations found in CIITA and RFX, organized 5' to 3' down the lengths of the genes. Bolded mutations indicate those which were interpreted as likely to affect expression or function of the mutated protein.
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In summary, mutations in RFX and CIITA were infrequent in MHCII-DLBCL. These cases were clinical samples with heterogeneous cell populations and thus it is possible that mutations were under-identified. However, the most likely mechanism for loss of MHCII expression in DLBCL remains specific, co-ordinated downregulation of MHCII transcription, which could potentially be restored through therapeutic intervention.
Footnotes
Funding: grant from the American Cancer Society, #RSG0605501L1B. The authors have no relevant conflicts of interest to disclose.
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