Haematologica, Vol 94, Issue 6, 745-747 doi:10.3324/haematol.2009.006965
Copyright © 2009 by Ferrata Storti Foundation
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Editorials and Perspectives

A role for the renin-angiotensin system in hematopoiesis

Tea Soon Park, Elias T. Zambidis

Institute for Cell Engineering, Stem Cell Program, Johns Hopkins University School of Medicine, and Division of Pediatric Oncology, Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD, USA, E-mail:ezambid1{at}jhmi.edu

The effects of the renin-angiotensin system (RAS) on blood pressure regulation were first described in 1898 by the physiologist Robert Tigerstedt, following observations that blood pressure increased in anesthetized animals following the injection of kidney extracts.1–3 Since then, the RAS has been characterized extensively, not only for its roles in hypertension and atherosclerosis, but also for its relevance to the fields of diabetes, oncology, and hematology. It has been recently recognized that the RAS plays critical physiological roles beyond its originally recognized functions in cardiovascular and fluid homeostasis regulation. For example, in this issue of the journal, Heringer-Walther et al.4 investigated the effects of angiotensin-(1–7) on in vitro proliferation and in vivo engraftment of human hematopoietic progenitor cells. These authors reported that the angiotensin II (Ang II) metabolite, Ang-(1–7) positively stimulates the proliferation of CD34+ cord blood cells in vitro. They also demonstrated that coinjection of cord blood mononuclear cells with continuous post-transplantation supplementation of Ang-(1–7) drastically improved human hematopoietic progenitor engraftment. These results continue to highlight an important yet unappreciated role for RAS in regulating hematopoietic progenitor differentiation and self-renewal. Moreover, the use of Ang-(1–7), Ang II, or manipulation of Ang II receptor binding may have utility and clinical significance in bone marrow transplantation.

The RAS pathway mediates its effects primarily via angiotensinogen, angiotensin I (Ang I), and Ang II. The enzyme renin converts angiotensinogen to Ang I, which is transformed into Ang II by the surface ectoenzyme angiotensin I-converting enzyme (ACE; CD143). Ang II alternatively binds to either Ang II-type 1 receptors (AGTR1, a.k.a., AT1), or angiotensin II-type 2 receptors (AGTR2, a.k.a., AT2).5 Although AGTR1 is expressed abundantly in renal and non-renal tissues from development to adulthood (where it functions as a key regulator of the cardiovascular system through G-protein-coupled interactions), AGTR2 is scarcely expressed in adult tissues,6,7 suggesting a key role during early development. The AGTR1 and AGTR2 can act as antagonists, and mediate affects on cell migration and proliferation of cardiovascular cells, metastatic cancer cells, and hematopoietic stem-progenitor cells (HSC).5,8 The regulation of the RAS on the hematopoietic system has been progressively documented over the last decade. In 1982, two independent groups first reported that high doses of ACE inhibitors could induce anemia and leucopenia in humans.9,10 Ang II receptor antagonists and ACE inhibitors are commonly used for treatment of cardiovascular diseases, post-transplantation erythrocytosis, or polycythemia vera.11 Nonetheless, the mechanisms by which RAS components and their inhibitors maintain balance in the hematopoietic and cardiovascular systems remain obscure.

ACE inhibitors and angiotensin receptor blockers are routinely used and clinically well-tolerated agents, despite sporadic reports of associated side effects that include severe anemia and bone marrow aplasia.12,13 A review of the literature suggests that the effects of the RAS on regulation of hematopoietic differentiation are numerous and diverse. Renin, angiotensinogen, ACE, and/or AGTR1 are expressed in human umbilical cord blood,14 bone marrow cells,15 and CD34+ HSC,13,16 and Ang II peptide can modulate erythropoiesis either by upregulating erythropoietin levels, or by exerting mitogenic effects on erythroid progenitors and CD34+ HSC. Furthermore, HSC can be differentiated toward the erythroid lineage by Ang II-AGTR1 interactions, and this effect can be abrogated by blocking Ang II binding using Losartan, an AGTR1 inhibitor.17 ACE was also shown to increase the recruitment of primitive HSC into S-phase in the bone marrow via degradation of the tetrapeptide Ac-SDKP (acetyl-seryl-aspartyl- lysyl-proline), a potent inhibitor of HSC proliferation.18,19 Aksu, et al. reported that surface ACE is overexpressed in leukemic myeloid blast cells, and such expression positively correlated with the number of bone marrow blast counts.20

Table 1 summarizes the most commonly used ACE inhibitors, and AGTR1/AGTR2 antagonists that are currently available for treatment of cardiovascular/renal disease, and their known effects on the hematopoietic system. Interestingly, treatment with AGTR1 antagonists in the adult resulted in side effects similar to those observed with ACE inhibitors (e.g. anemia), which was the opposite result obtained in human embryonic hematopoiesis.


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  		Table 1. Components of the RAS and summary of effects on the hematopoietic system.

Studies of knockout mice mutant for ACE (as well as other RAS components such as angiotensinogen, renin, AGTR1, and AGTR2) have further implicated a regulatory role for the RAS in hematopoiesis.23 These mice have exhibited not only phenotypes related to blood pressure, but have also demonstrated defects in development, as well as in cardiovascular, reproductive, and hematopoietic systems. ACE null mice are mildly anemic (exhibiting hematocrit levels of about 40% when compared with normal levels of about 50%), and are also characterized by increased levels of serum and urinary potassium secondary to kidney developmental defects. The mechanisms of this anemia remain unclear, although it is presumed that the lack of systemic or local production of Ang II has a detrimental effect on erythropoiesis.18,19,24,25 Interestingly, a mouse model (ACE 10/10 mice) with specific overexpression of ACE in monocytes and macrophages, but lacking expression in other cell types (including endothelium and renal tissues) displayed significantly reduced tumor vascularization.23 These studies suggested that abnormal ACE expression in hematopoietic cells can perturb angiogenesis mediated by myeloid cells.

The RAS has been further implicated in playing a critical role during the development of the hematopoietic system. The recent discovery that ACE (CD143) marks primitive human embryonic hemangioblasts21 has now raised the possibility that the RAS plays a critical role in regulating the earliest stages of human hematoendothelial differentiation, as it does in avian embryos.26 Not surprisingly, RAS components are locally expressed in all of the important sites of emerging angiohematopoiesis including the yolk sac, liver, kidney, embryonic aorta, and retinal/choroid regions.27 Accordingly, the hemangioblast, a common precursor for both hematopoietic and endothelial cells, is first observed in the yolk sac blood islands and aorta gonad mesonephros (AGM) during embryogenesis in the same regions where these RAS components are also first expressed; Jokubaitis et al. confirmed that ACE (CD143) is expressed in human embryonic, fetal, and adult hematopoietic organs including the ventral wall of the aorta, fetal liver, and umbilical cord blood.8

Using a human embryonic stem cell (hESC) differentiation model, we recently reported that there was a dramatic upregulation of AGTR2 during expansion of human embryoid body (hEB)-derived ACE+ hemangioblasts.21 This observation suggested a unique role for the RAS in guiding the initial developmental phases of human hemato-endotheliogenesis. Moreover, hESC-derived hemangioblast colonies could be directed to differentiate into either hematopoietic or endothelial progeny by manipulating the signaling pathways normally mediated by the RAS. Manipulation of angiotensin II signaling with either AGTR1- or AGTR2-specific inhibitors resulted in pronounced skewing of hEB differentiation toward either endothelium, or multipotent hematopoietic progenitors. Since AGTR2 signaling is known to antagonize AGTR1 signaling directly,28 these data implicated a general mechanism by which emerging hemangioblasts in the yolk sac or AGM may be directed to differentiate by the hematopoietic stem cell niche. One possibility is that antagonistic competition between AGTR1 and AGTR2 for Ang II binding on emerging hemangioblasts directs their development into either hematopoietic progenitors, or alternatively into vascularendothelial networks. Finally, these studies suggest that manipulation of AGTR2 signaling is an important mechanism by which multipotent hematopoietic progenitors expand from primitive HSC. Furthermore, it is also possible that AGTR1/AGTR2- regulated stem cell proliferation is a generalized phenomenon, since AGTR1 inhibition by losartan was reported to increase skeletal muscle regeneration in patients with primary muscular dystrophies,29 and the renin-angiotensin axis critically influences normal fetal development, since both ACE and AGTR1 receptor blockers are teratogenic. Additionally, AGTR2 signaling is known to regulate cellular growth and apoptosis during vascular and neural development.26,30

In summary, these studies collectively implicate the involvement of the RAS in a broad spectrum of functions that surpass its known functions in cardiovascular homeostasis. ACE and its major enzymatic product, Ang II are recognized as critical determinants of angiogenesis, inflammation, tumor progression, and hematopoiesis. Modulation of RAS function may be determined through competitive interaction of Ang II with two antagonistic receptors, AGTR1 and AGTR2, and this intrinsic bivalence may regulate the fate of emerging hemangioblasts (which are present in the embryonic yolk sac or the AGM), or the adult HSC found in the bone marrow. The levels of regulation involved here are likely to be extremely complex and multilayered. For example, ACE is actively degraded by the vasoactive peptide bradykinin, and as a consequence, ACE inhibitors can act both upstream (by interfering with Ang II production), as well as downstream via direct bradykinin effects on the RAS pathway. Additionally, ACE inhibitors with thiol groups can also directly inhibit the activities of zinc metalloproteases MMP2 and MMP9,5 which may connect the RAS to possible roles in migration of tumorigenic cells and HSC. A deeper understanding of how these multiple levels of the protean RAS regulate the development and differentiation of the hematopoietic system may provide new insights to help design improved therapies for hematologic disorders.

Footnotes

Dr. Zambidis is an Assistant Professor of Pediatrics and Oncology at the Johns Hopkins School of Medicine, Baltimore, MD, USA.

Dr. Park is a postdoctoral Research Fellow at the Institute for Cell Engineering, at the Johns Hopkins School of Medicine, Baltimore, MD, USA.

References

  1. Tigerstedt R, Bergman PG. Niere und Kreislauf. Scandinav Arch J Physiol 1898;8:223.
  2. Bernstein KE, Martin BM, Bernstein EA, Linton J, Striker L, Striker G. The isolation of angiotensinconverting enzyme cDNA. J Biol Chem 1988;263:11021-4.[Abstract/Free Full Text]
  3. Ferrario CM. Does angiotensin-(1–7) contribute to cardiac adaptation and preservation of endothelial function in heart failure?. Circulation 2002;105:1523-5.[Free Full Text]
  4. Heringer-Walther S, Eckert K, Schumacher S-M, Uharek L, Wulf-Goldenberg A, Gembardt F, et al. Angiotensin-(1–7) stimulates hematopoietic progenitor cells in vitro and in vivo. Haematologica 2009;94:857-60.[Abstract/Free Full Text]
  5. Deshayes F, Nahmias C. Angiotensin receptors: a new role in cancer?. Trends Endocrinol Metab 2005;16:293-9.[CrossRef][Web of Science][Medline]
  6. Grady EF, Sechi LA, Griffin CA, Schambelan M, Kalinyak JE. Expression of AT2 receptors in the developing rat fetus. J Clin Invest 1991;88:921-33.[Web of Science][Medline]
  7. Akishita M, Ito M, Lehtonen JY, Daviet L, Dzau VJ, Horiuchi M. Expression of the AT2 receptor developmentally programs extracellular signal-regulated kinase activity and influences fetal vascular growth. J Clin Invest 1999;103:63-71.[Web of Science][Medline]
  8. Jokubaitis VJ, Sinka L, Driessen R, Whitty G, Haylock DN, Bertoncello I, et al. Angiotensinconverting enzyme (CD143) marks hematopoietic stem cells in human embryonic, fetal, and adult hematopoietic tissues. Blood 2008;111:4055-63.[Abstract/Free Full Text]
  9. Studer A, Vetter W. Reversible leucopenia associated with angiotensin-converting-enzyme inhibitor MK 421. Lancet 1982;1:458.[Web of Science][Medline]
  10. Griffing GT, Melby JC. Enalapril (MK-421) and the white cell count and haematocrit. Lancet 1982;1:1361.[Web of Science][Medline]
  11. Marubayashi S, Yamamoto H, Shibata S, Fudaba Y, Miyata Y, Fukuma K, et al. Effect of the angiotensin-converting enzyme inhibitor enalapril on post-transplant erythrocytosis. Hiroshima J Med Sci 1998;47:121-4.[Medline]
  12. Mrug M, Stopka T, Julian BA, Prchal JF, Prchal JT. Angiotensin II stimulates proliferation of normal early erythroid progenitors. J Clin Invest 1997;100:2310-4.[Web of Science][Medline]
  13. Rodgers KE, Xiong S, Steer R, di Zerega GS. Effect of angiotensin II on hematopoietic progenitor cell proliferation. Stem Cells 2000;18:287-94.[CrossRef][Web of Science][Medline]
  14. Goker H, Haznedaroglu IC, Beyazit Y, Aksu S, Tuncer S, Misirlioglu M, et al. Local umbilical cord blood renin-angiotensin system. Ann Hematol 2005;84:277-81.[CrossRef][Web of Science][Medline]
  15. Haznedaroglu IC, Tuncer S, Gursoy M. A local renin- angiotensin system in the bone marrow. Med Hypotheses 1996;46:507-10.[CrossRef][Web of Science][Medline]
  16. Haznedaroglu IC, Ozturk MA. Towards the understanding of the local hematopoietic bone marrow renin-angiotensin system. Int J Biochem Cell Biol 2003;35:867-80.[CrossRef][Web of Science][Medline]
  17. Kato H, Ishida J, Imagawa S, Saito T, Suzuki N, Matsuoka T, et al. Enhanced erythropoiesis mediated by activation of the renin-angiotensin system via angiotensin II type 1a receptor. FASEB J 2005;19:2023-5.[Abstract/Free Full Text]
  18. Li J, Volkov L, Comte L, Herve P, Praloran V, Charbord P. Production and consumption of the tetrapeptide AcSDKP, a negative regulator of hematopoietic stem cells, by hematopoietic microenvironmental cells. Exp Hematol 1997;25:140-6.[Web of Science][Medline]
  19. Gaudron S, Grillon C, Thierry J, Riches A, Wierenga PK, Wdzieczak-Bakala J. In vitro effect of acetyl-N-Ser-Asp-Lys-Pro (AcSDKP) analogs resistant to angiotensin I-converting enzyme on hematopoietic stem cell and progenitor cell proliferation. Stem Cells 1999;17:100-6.[Web of Science][Medline]
  20. Aksu S, Beyazit Y, Haznedaroglu IC, Canpinar H, Kekilli M, Uner A, et al. Over-expression of angiotensin-converting enzyme (CD 143) on leukemic blasts as a clue for the activated local bone marrow RAS in AML. Leuk Lymphoma 2006;47:891-6.[CrossRef][Web of Science][Medline]
  21. Zambidis ET, Park TS, Yu W, Tam A, Levine M, Yuan X, et al. Expression of angiotensin-converting enzyme (CD143) identifies and regulates primitive hemangioblasts derived from human pluripotent stem cells. Blood 2008;112:3601-14.[Abstract/Free Full Text]
  22. Rousseau-Plasse A, Wdzieczak-Bakala J, Lenfant M, Ezan E, Genet R, Robinson S, et al. Lisinopril, an angiotensin I-converting enzyme inhibitor, prevents entry of murine hematopoietic stem cells into the cell cycle after irradiation in vivo. Exp Hematol 1998;26:1074-9.[Web of Science][Medline]
  23. Shen XZ, Xiao HD, Li P, Billet S, Lin CX, Fuchs S, et al. Tissue specific expression of angiotensin converting enzyme: a new way to study an old friend. Int Immuno-pharmacol 2008;8:171-6.[CrossRef][Web of Science][Medline]
  24. Cole J, Ertoy D, Lin H, Sutliff RL, Ezan E, Guyene TT, et al. Lack of angiotensin II-facilitated erythropoiesis causes anemia in angiotensin-converting enzyme-deficient mice. J Clin Invest 2000;106:1391-8.[Web of Science][Medline]
  25. Xiao HD, Fuchs S, Frenzel K, Teng L, Li P, Shen XZ, et al. The use of knockout mouse technology to achieve tissue selective expression of angiotensin converting enzyme. J Mol Cell Cardiol 2004;36:781-9.[CrossRef][Web of Science][Medline]
  26. Savary K, Michaud A, Favier J, Larger E, Corvol P, Gasc JM. Role of the renin-angiotensin system in primitive erythropoiesis in the chick embryo. Blood 2005;105:103-10.[Abstract/Free Full Text]
  27. Schutz S, Le Moullec JM, Corvol P, Gasc JM. Early expression of all the components of the reninangiotensin- system in human development. Am J Pathol 1996;149:2067-79.[Abstract]
  28. Hubert C, Savary K, Gasc JM, Corvol P. The hematopoietic system: a new niche for the reninangiotensin system. Nat Clin Pract Cardiovasc Med 2006;3:80-5.[CrossRef][Web of Science][Medline]
  29. Cohn RD, van Erp C, Habashi JP, Soleimani AA, Klein EC, Lisi MT, et al. Angiotensin II type 1 receptor blockade attenuates TGF-beta-induced failure of muscle regeneration in multiple myopathic states. Nat med 2007;13:204-10.[CrossRef][Web of Science][Medline]
  30. Grammatopoulos TN, Ahmadi F, Jones SM, Fariss MW, Weyhenmeyer JA, Zawada WM. Angiotensin II protects cultured midbrain dopaminergic neurons against rotenone-induced cell death. Brain res 2005;1045:64-71.[Web of Science][Medline]

Related Article

Angiotensin-(1–7) stimulates hematopoietic progenitor cells in vitro and in vivo
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Haematologica 2009 94: 857-860. [Abstract] [Full Text] [PDF]




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