- Haiwei Lian1,2,3,
- Dun Li2,4,
- Yun Zhou2,5,
- Esther Landesman-Bollag4,
- Guanglan Zhang6,
- Nicole M. Anderson2,
- Kevin Charles Tang2,
- Justine E. Roderick7,
- Michelle A. Kelliher7,
- David C. Seldin4,
- Hui Fu1⇑ and
- Hui Feng2,4⇑
- 1Department of Anatomy and Embryology, Wuhan University School of Basic Medical Sciences, Wuhan, Hubei, P.R. China
- 2Department of Pharmacology & Experimental Therapeutics, Boston University School of Medicine, MA, USA
- 3Department of Neurosurgery, Wuhan University Renmin Hospital, Wuhan, Hubei, P.R. China
- 4Department of Medicine, Section of Hematology and Medical Oncology, Boston University School of Medicine, MA, USA
- 5Department of Gynaecology, Wuhan University Renmin Hospital, Wuhan, Hubei, P.R. China
- 6Department of Computer Science, Metropolitan College, Boston University, MA, USA
- 7Department of Molecular, Cell and Cancer Biology, University of Massachusetts School of Medicine, Worcester, MA, USA
- Correspondence: /
T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive cancer of developing thymocytes, and remains fatal in 20% of pediatric and 50% of adult patients.1,2 Frequent application of multi-agent cytotoxic drugs leads to disease relapse and high toxicities, underscoring the need for targeted therapies. The suppression of aberrant NOTCH1 signaling in T-ALL cells by gamma secretase inhibitors (GSIs) has been met with much enthusiasm; however, the gastrointestinal toxicities and drug resistance of GSIs restrain their clinical applications.3 The proto-oncogene MYC is a transcriptional target of NOTCH1 and a dominant driver of T-ALL pathogenesis.3 Targeting MYC-mediated transcriptional programs through BET bromodomain inhibitor JQ1 exhibits anti-leukemic efficacy in vitro and in vivo.4 However, global repression of transcription is predicted to cause toxicities. Identification of drug(s) synergizing with JQ1 to kill T-ALL cells may enhance the efficacy while reducing toxicities. Protein kinase CK2 is a tetrameric serine-threonine kinase composed of two catalytic (α or α′) and regulatory (β) subunits that can phosphorylate NOTCH1.5 CK2 inhibition by CX-4945, a potent and specific inhibitor in clinical trials for treating breast cancer and multiple myeloma, significantly reduces growth and survival of human T-ALL cells,6 and down-regulates NOTCH1 in lung cancer cells.7 However, it remains unclear whether the cytotoxic effect of CX-4945 on T-ALL cells is associated with repression of NOTCH1 signaling. Here we show that CK2 inhibition by CX-4945 destabilizes NOTCH1 and synergizes with JQ1 to induce apoptosis in human T-ALL cells, implicating an alternative strategy to target NOTCH1 signaling in refractory/relapsed T-ALL.
CK2 (α and β) was previously found up-regulated in human T-ALL cells;8 whether this upregulation is linked to the temporal regulation of CK2 during T-cell development is unknown. To address this question, we analyzed publicly available databases and cross-compared the expression of CK2 subunits among subsets of developing T cells and patient T-ALL cells that are arrested at different developmental stages.9 We found that the transcript levels of all CK2 subunits (α, α′ and β) were significantly higher in patient T-ALL cells, compared to normal T cells regardless of their developmental stages (Figure 1A and B and Online Supplementary Figure S1A). We next examined protein levels of CK2, cleaved-NOTCH1 and MYC by Western blots in a panel of primary T-ALL patient samples. Consistent with its elevated transcript levels, CK2α′ protein levels were up-regulated in patient T-ALL cells, compared with those in normal thymus (Figure 1C and D). While CK2α protein levels were not significantly elevated in the patient samples examined (Figure 1C and Online Supplementary Figure S1B), they significantly correlated with those of cleaved-NOTCH1 and MYC (Figure 1E and F). In addition, we observed significant correlation between protein levels of CK2α′ and cleaved-NOTCH1 (Figure 1G). Western blotting analysis of CK2α, CK2α′, CK2β, cleaved-NOTCH1 and MYC in a panel of human T-ALL cell lines (JURKAT, ALL-SIL, RPMI-8402 and MOLT-3) revealed that human T-ALL cells expressed significantly higher levels of CK2α′, NOTCH1 and MYC, compared with those in normal thymus (Online Supplementary Figure S2). These results demonstrate that CK2, the α′ subunit in particular, is aberrantly expressed in human T-ALL cells regardless of their stages of differentiation blockade, and its expression correlates with cleaved-NOTCH1 and MYC.
To understand whether CX-4945 can modulate NOTCH1 signaling in the context of T-ALL cells, we treated JURKAT and ALL-SIL cells that up-regulate all three subunits of CK2, as well as RPMI-8402 cells expressing much less CK2, with CX-4945. CK2 enzymatic activity was efficiently inhibited by CX-4945 treatment in these cells, as demonstrated by decreased levels of phospho-AKT serine 129 (Figure 2A and Online Supplementary Figure S3B),10 as well as CK2 kinase assays (Online Supplementary Figure S3A). Interestingly, CK2 inhibition by CX-4945 led to a dose-dependent decrease of cleaved-NOTCH1 in JURKAT and ALL-SIL cells as early as 8 hours post treatment (Figure 2A). The effect of CX-4945 on cleaved-NOTCH1 was less obvious in RPMI-8402 cells, consistent with low expression of CK2 in this cell line (Online Supplementary Figure S2B). To understand why CX-4945 treatment reduced NOTCH1 levels, we blocked proteasome-mediated degradation in JURKAT and ALL-SIL cells with the proteasome inhibitor MG132. Because JURKAT cells are exquisitely sensitive to MG132 treatment, rapidly inducing apoptosis,11 we performed this experiment in JURKAT cells that over-express BCL-2 and thus are apoptotic-resistant. In the absence of CX-4945, MG132 treatment led to increased NOTCH1 levels in both T-ALL cell lines, indicating that NOTCH1 is degraded by the proteasome in these cells (Figure 2B). Importantly, MG132 treatment restored cleaved-NOTCH1 levels that declined upon CX-4945 treatment in both cell lines (Figure 2B), suggesting that CX-4945 treatment promotes the degradation of NOTCH1 in these cells. Pulse-chase analysis was subsequently performed to measure the half-life of cleaved-NOTCH1 in JURKAT and ALL-SIL T-ALL cells with and without CX-4945 treatment (Figure 2C), and revealed that NOTCH1 was degraded at least twice as fast in TALL cells upon CX-4945 treatment, compared to control DMSO-treated cells (Figure 2C).
Because MYC is a direct transcriptional target of NOTCH1 and critical for T-ALL pathogenesis,3 we next examined the extent to which CX-4945 treatment decreases MYC transcript levels through quantitative RT-PCR analysis. Compared with DMSO-treated T-ALL cells, CX-4945 treatment led to a significant reduction of MYC transcripts as early as eight hours post treatment (Figure 2D and additional data not shown). To preclude the possible effects of apoptosis on overall protein levels, we treated JURKAT BCL-2-over-expressing cells that are apoptotic-resistant with CX-4945, and observed drastic reductions of both cleaved-NOTCH1 and MYC protein levels after 24 hours of treatment (Figure 2E). These data demonstrate that CX-4945 treatment destabilizes NOTCH1, leading to subsequent downregulation of MYC transcript and protein levels.
Because both JQ1 and CX-4945 exhibit anti-leukemic efficacy as single agents,4,6 and our results show that CX-4945 destabilizes NOTCH1 and down-regulates MYC in T-ALL cells, we next asked whether CX-4945 synergizes with JQ1 to kill human T-ALL cells. To this end, we treated JURKAT, ALL-SLL, RPMI-8402 and MOLT-3 cells with serial dilutions of CX-4945 and JQ1 in combination and analyzed relative cell viability. In both JURKAT and ALL-SIL cells that significantly up-regulate CK2 subunits (CK2α, CK2α′ and CK2β; see Online Supplementary Figure S2A), CX-4945 and JQ1 exhibited strong synergism, with an average combination index (CI) of 0.31 and 0.16, respectively (Figure 3A and B; where a CI of 1 indicates an additive effect, CI<1 is synergistic, and CI>1 antagonistic). However, for RPMI-8402 and MOLT-3 cells, which express moderate levels of CK2β (Online Supplementary Figure S2A), the synergism is weaker, with an average CI of 0.49 and 0.75, respectively (Figure 3A and B). Our data demonstrate that T-ALL cells, especially those with CK2 upregulation, are more sensitive to the combination treatment of CX-4945 and JQ1 than to single agents.
To understand the cellular basis for the synergism of CX-4945 and JQ1 in T-ALL cells, we performed apoptosis and cell cycle analyses. We used Annexin V and propidium iodide (PI) staining to document that the combination treatment of CX-4945 (2.5 μM) and JQ1 (1 μM) in our tested four T-ALL cells significantly induced apoptosis, compared with either drug alone (Figure 3C and Online Supplementary Figure S4). Furthermore, Western blotting analysis showed much stronger expression of cleaved-PARP in human T-ALL cells subjected to combination treatment than those in single-agent-treated cells (Online Supplementary Figure S5). Finally, cell cycle analysis of T-ALL cells failed to reveal cell cycle arrest upon single or combination treatment (data not shown), indicating that apoptosis is the primary cellular basis of synergism.
To determine the possible toxicities of the combination treatment, we treated ALL-SIL T-ALL cells and normal peripheral blood monocytes (PBMC) with a higher dosage of CX-4945 (5 μM) and JQ1 (2 μM) than those used in Figure 3B and C, alone or in combination for 48 hours. Importantly, PBMC were less sensitive to CX-4945, JQ1, or combination treatment, compared with ALL-SIL T-ALL cells (Online Supplementary Figure S6A). Moreover, when we tested the combination treatment in PBMC, we observed an antagonistic effect (CI=1.11) (Online Supplementary Figure S6B).
In conclusion, our studies show that CK2 inhibitor CX-4945 destabilizes NOTCH1 and synergizes with JQ1 against human T-ALL cells. Both FBW7 and ITCH can degrade NOTCH1,12 and ITCH may promote NOTCH1 degradation in T-ALL cells (e.g. JURKAT) with FBW7 mutations.13 Importantly, CX-4945 exhibits striking synergy with JQ1 in T-ALL cells that up-regulate CK2, cleaved-NOTCH1 and MYC. CX-4945 induces proapoptotic unfolded protein response (UPR) in T-ALL cells,6 while JQ1 down-regulates MYC that normally activates prosurvival UPR.14 Hence, CX-4945 and JQ1 may synergistically kill T-ALL cells by enabling the switch of prosurvival to proapoptotic UPR. Although JQ1 can also synergize with the GSI to kill T-ALL cells,15 due to the toxicities and drug resistance of GSI, the combination of CX-4945 and JQ1 may offer a better approach to target NOTCH1 signaling in refractory/relapsed T-ALL. Both CX-4945 and JQ1 structural analogs are currently in clinical trials as single agents to treat solid and hematologic cancers (clinicaltrials.gov identifiers: 02128282 and 02157636). Our studies provide a rationale to test the combination of CX-4945 and JQ1 on refractory/relapsed T-ALL using pre-clinical in vivo models. Our data from TALL cell lines suggest that patient T-ALL cells with elevated CK2 expression could be more sensitive to the treatment than those with low CK2 expression. Given the wide involvement of CK2 and NOTCH1/MYC in cancers, the combination treatment of JQ1 and CX-4945 should be investigated in other cancer types.
Information on authorship, contributions, and financial & other disclosures was provided by the authors and is available with the online version of this article at www.haematologica.org.
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