Author Affiliations
- Valentina Sangiorgio1⇑,
- Luis Veloza2,
- Karen Galvis3,
- Mónica López2,
- Gerard Frigola2,
- Elias Campo2 and
- Olga Balagué2
- 1Department of Cellular Pathology, Barts and The London NHS Trust, London, UK
- 2Haematopathology Unit, Pathology Department, Hospital Clinic of Barcelona, University of Barcelona, Villarroel, Barcelona, Spain and
- 3Department of Pathology and Laboratory Medicine, Fundación Santa Fe de Bogotá University Hospital, Bogotá, Colombia
- Correspondence: VALENTINA SANGIORGIO: valentina.sangiorgio{at}nhs.net
Data supplements
Disclosures and Contributions
Files in this Data Supplement:
ARTICLE FIGURES & DATA
Figures
- Figure 1.
Histopathological and immunophenotypical features of the two Kikuchi-Fujimoto disease cases. A partial effacement of the architecture, with an expansion of the paracortex, pale areas of necrosis (black arrows) and residual lymphoid follicles are seen in both cases (A, H&E). The necrotic areas contain abundant apoptotic debris, numerous histiocytes, some with reniform nuclei, so called “crescentic histiocytes” (black arrows) (B, H&E). Both cases show an abundant population of CD3-positive T cells (C, CD3 IHC), with a predominance of the CD8 (D, CD8 IHC) over CD4-positive T-cells (E, CD4 IHC). Case 1 shows a partial loss (<50%) of CD5 (F, CD5 IHC). Abundant granzyme B-positive cytotoxic cells are identified in both cases (G, granzyme B IHC). Histiocytes express CD163 (H, CD163 IHC), with cytoplasmic co-expression of myeloperoxidase (I, myeloperoxidase IHC) and PD-L1 (J, PD-L1 IHC). Aggregates and sheets of plasmacytoid dendritic cells are observed in both cases (K, H&E), which are positive for CD123 (L, CD123 IHC). Original magnifications: A: 1.25x; B, K: 40x; C-J, L: 20x.
- Figure 2.
CD30-positive atypical cells in case 1. Case 1 showed numerous interfollicular clusters of CD30-positive atypical cells also surrounding the necrotic areas (A, H&E). CD30+ atypical intermediate/large cells show ample cytoplasm and some prominent nucleoli (B, H&E). Composite image of double immunostainings with CD30 (brown) and granzyme B (red) demonstrated that most CD30+ atypical cells co-expressed granzyme B indicating that they corresponded to activated cytotoxic T cells (black arrows) (C, CD30 and granzyme B, double IHC), while they did not show co-expression of PAX5 (red) (black arrows) (D, CD30 and PAX5, double IHC). Tbet transcription factor (red) was positive in a proportion of CD30-positive cells (brown) (inset in D, CD30 and Tbet, double IHC). Original magnifications: A: 10x; B-D: 40x.
- Figure 3.
T-cell receptor rearrangement studies in case 1. Both the original sample, diagnosed as ALCL in another institution (A) and the new sample, suspicious for relapse (B) were polyclonal for TCRγ gene rearrangements. TCRß gene rearrangement study was only performed in the second sample due to poor quality DNA (C).
Vol 104 Issue 12
