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Molecular diagnosis of leukemic cerebrospinal fluid cells in children with newly diagnosed acute lymphoblastic leukemia
CA Scrideli, RP Queiroz, OM Takayanagui, JE Bernardes, EV Melo, LG Tone
Haematologica January 2004 89: 1013-1015; doi:
CA Scrideli
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RP Queiroz
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OM Takayanagui
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JE Bernardes
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EV Melo
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LG Tone
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Author Affiliations

  1. CA Scrideli,
  2. RP Queiroz,
  3. OM Takayanagui,
  4. JE Bernardes,
  5. EV Melo and
  6. LG Tone
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Abstract

Cytomorphology and IgH/T-cell receptor g clonal gene rearrangements detected by polymerase chain reaction (PCR) homo/heteroduplex analysis and direct sequencing were evaluated in cerebrospinal fluid (CSF) free of red-blood cells at diagnosis of 37 children with acute lymphoblastic leukemia. Molecular CSF involvement was greater as detected by molecular analysis than observed by morphologic criteria (45.9% vs 5.4%). The 4-year event-free survival was lower in the group with molecularly detected CSF involvement (p = 0.01).

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Vol 89 Issue 8

Haematologica: 89 (8)
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Molecular diagnosis of leukemic cerebrospinal fluid cells in children with newly diagnosed acute lymphoblastic leukemia
CA Scrideli, RP Queiroz, OM Takayanagui, JE Bernardes, EV Melo, LG Tone
Haematologica Jan 2004, 89 (8) 1013-1015;

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CA Scrideli, RP Queiroz, OM Takayanagui, JE Bernardes, EV Melo, LG Tone
Haematologica Jan 2004, 89 (8) 1013-1015;
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