Methodological approach to minimal residual disease detection by flow cytometry in adult B-lineage acute lymphoblastic leukemia
M Krampera, O Perbellini, C Vincenzi, F Zampieri, A Pasini, MT Scupoli, A Guarini, MS De Propris, E Coustan-Smith, D Campana, R Foa, G Pizzolo

Author Affiliations

  1. M Krampera,
  2. O Perbellini,
  3. C Vincenzi,
  4. F Zampieri,
  5. A Pasini,
  6. MT Scupoli,
  7. A Guarini,
  8. MS De Propris,
  9. E Coustan-Smith,
  10. D Campana,
  11. R Foa and
  12. G Pizzolo
  1. Dipartimento di Medicina Clinica e Sperimentale, Sezione di Ematologia, Universita di Verona. mauro.krampera@univr.it

Abstract

A flow cytometric approach to minimal residual disease (MRD) monitoring useful in childhood B-lineage acute lymphoblastic leukemia (ALL) is discussed here in the context of ALL in adults. Of 64 leukemia samples analyzed, 95.3% had at least one abnormal phenotype (57.3% had two or more) as compared to physiologic B-cell precursors in adult bone marrow. The method was sensitive enough to detect one leukemic cell among 10,000 normal cells in 16/19 experiments (84.2%). Blast phenotypes were stable in culture and at relapse, and were useful for MRD monitoring in patients. Marker combinations for childhood ALL are also applicable to adult cases.