- Lee Macpherson1,
- James Monypenny2,
- Michael P. Blundell3,
- Giles O. Cory4,
- Jessica Tomé-García1,
- Adrian J. Thrasher3,5,
- Gareth E. Jones2 and
- Yolanda Calle1⇓
- 1Department of Haemato-oncology, King's College London, London
- 2Randall Division of Cell and Molecular Biophysics, King's College London, London
- 3Centre for Immunodeficiency, Molecular Immunology Unit, Institute of Child Health, University College London
- 4Peninsula College of Medicine and Dentistry, Exeter
- 5Great Ormond Street Hospital NHS Trust, London, UK
- Correspondence: Dr. Yolanda Calle, Telephone: +18.104.22.168485816; Fax:+22.214.171.124333877; E-mail:
Podosomes are actin-based adhesions involved in migration of cells that have to cross tissue boundaries such as myeloid cells. The Wiskott Aldrich Syndrome Protein regulates de novo actin polymerization during podosome formation and it is cleaved by the protease calpain during podosome disassembly. The mechanisms that may induce the Wiskott Aldrich Syndrome Protein cleavage by calpain remain undetermined. We now report that in myeloid cells, tyrosine phosphorylation of the Wiskott Aldrich Syndrome Protein-tyrosine291 (Human)/tyrosine293 (mouse) not only enhances Wiskott Aldrich Syndrome Protein-mediated actin polymerization but also promotes its calpain-dependent degradation during podosome disassembly. We also show that activation of the Wiskott Aldrich Syndrome Protein leading to podosome formation occurs independently of tyrosine phosphorylation in spleen-derived dendritic cells. We conclude that tyrosine phosphorylation of the Wiskott Aldrich Syndrome Protein integrates dynamics of actin and cell adhesion proteins during podosome disassembly required for mobilization of myeloid cells during the immune response.
Complex patterns of adhesion and migration of leukocytes are required during the immune response. Cells of the myeloid lineage assemble characteristic highly dynamic actin-based adhesive structures termed podosomes that are thought to be involved in migration of cells that have to cross and invade tissue boundaries.1–2 Podosomes cluster behind the extending leading edge of migrating myeloid cells and are required for polarization, persistent migration and chemo-taxis.3–5 We and others have identified the Wiskott Aldrich Syndrome Protein (WASP) and the WASP interacting protein (WIP) in the podosome actin core4,6–8 where they play a major role in their formation and dynamics.4,8 WASP is expressed in hematopoietic cells and belongs to the N-WASP/WAVE family of adaptor proteins that mediate de novo actin polymerization. Inactive WASP exists in a hairpin autoinhibited conformation provided by the interaction between the Cdc42-binding domain (CRIB domain) and the VCA domain9 that can be relieved by binding to numerous upstream activators,9 including tyrosine kinases that phosphorylate (human) WASP Y291.10–11 Mutations in the gene coding for WASP lead to the Wiskott-Aldrich Syndrome (WAS), a hematologic disorder characterized by eczema, microthrombocytopenia, and immunodeficiency.12 WASP-deficient leukocytes exhibit defects in the organization and regulation of the actin cytoskeleton including the loss of podosomes.1–2 A point mutation (I294T) in WASP results in X-linked neutropenia13 similarly to at least two other activating WASP mutations also localized within the CRIB domain.13–14 The WASP I294T mutation resulted in enhanced actin polymerizing activity of WASP13 and macrophages expressing WASP I294T displayed an increased number of podosomes with enhanced rate of turnover.13 These results suggest that the active open conformation of WASP promotes actin polymerization as well as podosome disassembly. Although the idea that the same open conformation of WASP leads to both assembly and disassembly of podosomes may be counterintuitive at first, it is possible that for termination of podosomes actin polymerization and integrin recruitment have to be discontinued, and the same constituents of growing podosomes, such as WASP, may contribute to the disassembly process. We have previously shown that the rapid turnover of podosomes involves cleavage of WASP by the protease calpain,15 further supporting a role of WASP in both podosome formation and disassembly.1–2 However, the specific signaling mechanisms that make active WASP susceptible to cleavage by calpain leading to podosome disassembly remain unknown. In the present study, we address the possible role of phosphorylation of WASP Y291 in the calpain-mediated cleavage of WASP during podosome turnover in migrating immature dendritic cells (DCs).
Design and Methods
Pathogen free C57 mice purchased from Harlan and WASP-null, WASP Y293E and WASP Y293F expressing mice on a C57 background were bred in our own animal facility in pathogen free conditions. All animals were handled in strict accordance with good animal practice as defined by UK Home Office Animal Welfare Legislation, and all animal work was approved by the Institutional Research Ethics Committee (Institute of Child Health, University College London, UK) and performed under project licence number 70/7024.
DCs were generated from mouse spleens from wild-type, WASP-null, WASP Y293E and WASP Y293F expressing mice16 as previously described.8,15,17 THP-1 cells were obtained from the European Collection of Cell Cultures. Cells were infected using lentiviral vectors as previously described.3,15
Immunofluorescence and analysis of live microscopy
Cells were fixed and processed for immunofluorescence labeling as previously described.15 We used interference reflection microscopy (IRM) to visualize and analyze the adhesion-substratum interface of living cells using Mathematica™ 5.2 notebooks as previously described.15,18
Further details of methods used are described in the Online Supplementary Appendix.
Results and Discussion
Phosphorylation of WASP Y291 regulates the spatio-temporal dynamics of the podosome cluster
We have previously shown that expression of eGFP-tagged human WASP can recover the normal cytoskeletal organization and migratory properties of both human and mouse WASP null cells.3,15 In order to study the role of tyrosine phosphorylation of WASP in the formation and dynamics of individual podosomes, we expressed human eGFP-WASP or WASP phosphovariants in WASP −/−mouse spleen derived DCs. As expected, podosome clusters in WASP −/− DCs expressing wild-type WASP displayed treadmill dynamics with podosomes located at the back disassembling over time3,15 (Figure 1A, red pixels Figure 1E, light gray pixels, Figure 1H and Online Supplementary Video 1), newly formed podosomes assembled towards the margin of the leading edge (Figure 1A, blue pixels Figure 1E, light gray pixels Figure 1H) and a more stable central region that sustained the leading edge (yellow, pink and light blue pixels Figure 1E, dark gray and black pixels Figure 1H).8,15 In DCs expressing WASP Y291E (human phosphomimetic mutant), podosomes were of smaller size with a shorter life span (Figure 1B and D, Online Supplementary Video 2). Assembly and breakdown of podosomes occurred more rapidly and also persistently in the same position (white, light blue and pink pixels Figure 1F) and newly assembled podosomes located more randomly within the podosome cluster (blue pixels Figure 1F). As a result, the spatial shift of the podosome cluster towards the margin of the leading edge was impaired resulting in stabilization of the overall cell contact to the substratum (dark gray and black pixels in Figure 1I and K).16 Equivalent overall cell contact dynamics were observed previously in mouse cells expressing the mouse homologue mutants WASP Y293F/E.16 The finding that phosphorylation of WASP Y291 reduces the life-span of podosomes suggests that relieving the closed conformation of WASP by phosphorylation of WASP Y291 promotes both the previously reported enhanced actin polymerization10 as well as podosome disassembly similarly to the WASP I294T mutant.13 DCs expressing WASP Y291F (human phosphodead mutant) formed podosomes at the front of the cluster as the leading edge extended (Figure 1C, green pixels Figure 1G and Online Supplementary Video 3). However, podosomes remained in the same position for a longer period of time while increasing in size (Figure 1D and G) resulting in delayed disassembly and stabilization of the overall adhesion16 (dark gray and black pixels Figure 1J and K). Dynamics of eGFP-WASP proteins show that although both phosphomimetic and phosphodead mutations of WASP Y291 lead to stabilization of the overall cell adhesion, the actual dynamics of podosome cores (which are driven by WASP where it co-localizes with actin8) are different. This abnormal behavior of podosomes in cells expressing the WASP phosphomutants could explain the previously described abnormal matrix degradation, actin dynamics19 and impaired chemotaxis.16,19 Malfunction of these fundamental processes required for cells to trespass tissue barriers correlate with failure of mouse DCs expressing WASP phosphomutants to migrate across an endothelial cell monolayer similarly to WASP −/− cells (Figure 1L). Our results also show that WASP activation leading to podosome formation is independent of phosphorylation in Y291 in spleen-derived DC similarly to macrophages.19 However, we have previously reported that in bone marrow derived DCs, expression of the phosphodead variant of WASP resulted in formation of smaller podosomes predominantly.16 It is possible that the signaling mediated by specific posttranslational modifications of WASP may be dependent on the expression and/or activation of other pools of proteins capable of interacting with WASP expressed in specific cell subsets (myeloid cells from different sub-lineages or originated from different organs) or in activated cells.20 These specific interactions may result in different cytoskeletal regulation, which may reflect cell-specific routes of migration in vivo.
In summary, our results show that phosphorylation of WASP Y291 is not a limiting factor for podosome formation and is tightly regulated during the spatio-temporal dynamics of the podosome cluster facilitating spleen-derived DC podosome disassembly.
Phosphorylation of WASP Y293 promotes calpain mediated podosome disassembly
As expected, WASP Y293E (mouse phosphomimetic mutant) and WASP Y293F (mouse phosphodead mutant) spleen-derived DCs formed podosomes with smaller and larger actin cores, respectively, in comparison to WT cells (Figure 2A). In agreement with our previous results in DCs expressing wild-type WASP15 (Online Supplementary Figure S1A and D), blocking calpain activity in WASP Y293E expressing DCs resulted in increased size of podosome actin core and accumulation of vinculin (Figure 2A, Online Supplementary Figure S1B and E). The same treatments of WASP Y293F expressing DCs resulted in small increases in podosome size (Figure 2A, Online Supplementary Figure S1C and F) most likely as a result of inhibition of degradation of other podosomal components, such as talin, whose calpain-mediated cleavage also occurs during the turnover of podosomes in DCs.15 Inhibition of calpain did not induce changes in the percentage of DCs with podosomes (Figure 2B) but enhanced podosome stability (Figure 2C). These results indicate that the small size and enhanced turnover of podosomes in DCs expressing phosphomimetic WASP involve calpain activity. Mouse spleen-derived DCs expressing WASP -Y293E showed very limited expression of WASP (Figure 2D). Inhibition of calpain recovered the levels of WASP in WASP Y293E expressing DCs (Figure 2E) indicating that in the case of DCs, degradation of phosphorylated WASP is mediated predominantly by calpain rather than the proteasome. We have also described the preferential degradation of WASP by calpain rather than the proteasome in spleen-derived DCs in the absence of WIP.8 However, in other cells such as in splenocytes,16 WASP levels are modulated by the proteasome and not calpain suggesting that WASP stability may be preferentially regulated by different degradation systems depending on the cell type.16 Additionally, specific states of cell activation can also shift the pathway of cleavage/degradation of WASP. T cells, for example, degrade WASP via the proteasome under resting conditions whereas activation of T cells with PHA/IL-2 activates calpain and this pathway is preferentially used to degrade WASP.20 In spleen-derived DCs, the increased levels of full length WASP Y293E and the reduced cleavage of talin as a result of calpain inhibition15 (Figure 2E) may promote localized Arp2/3-mediated actin polymerization in the actin core and accumulation of paxillin and vinculin21 in the podosome ring, respectively. This accumulation of components may stabilize the anchorage between F-actin and integrins, resulting in enhanced podosome structural stability and the observed decrease in podosome turnover (Figure 2C).
Human WASP Y291E was also more susceptible to degradation, as shown in experiments with the human monocytic cell line THP-1 infected with lentiviral vectors coding for eGFP-WASP/WASP phosphovariants and WIP-mCherry.2 THP-1 cells expressed very low levels of eGFP-WASP alone (data not shown). The co-expresssion with WIP-mCherry was required for THP-1 cells to express easily detectable levels of eGFP-WASP and phosphovariants. Expression of WIP-mCherry stabilized and increased levels of both endogenous WASP and eGFP-WASP (Figure 2F). In THP-1 cells expressing the same levels of WIP-mCherry, the measured levels of eGFP-WASP Y291E were always lower than levels of eGFP-WASP or eGFP-WASP Y291F, independently of the multiplicity of infection (MOI) used (Figure 2F). We conclude that tyrosine phosphorylation not only promotes a sustained WASP open conformation10–11,16,22 but also enhances its susceptibility to calpain-mediated cleavage, thereby promoting disassembly of DC podosomes as the leading edge progresses.
The major role of WASP in myeloid cell biology is likely to be related to regulation of cell migration and other functions that require remodeling of the actin cytoskeleton rather than the control of cell differentiation. Although differentiation and proliferation of lymphoid cells is compromised by abnormal phosphorylation or lack of WASP,16 numbers of myeloid cells in vivo16,23 or expression of surface markers during DC maturation24 remained unaltered suggesting that WASP is not involved in differentiation of myeloid cells. Previous work15–16,24 and data shown in this study indicate a crucial role of WASP in organization of cell adhesion molecules and F-actin in DCs. These are fundamental processes also involved in phagocytosis,16 which is essential during the immune response mediated by macrophages and DCs. Our current data also show that the tight regulation of WASP phosphorylation regulates the effective migration of DC across tissue barriers such as endothelial cells and may be involved in the control of specific routes of migration of myeloid cells during the immune surveillance.23 Abnormal regulation of these functions in myeloid cells lacking WASP is thought to contribute to the phenotype of the Wiskott Aldrich Syndrome patients.
Taken together, our findings support the key role of WASP as a protein that integrates dynamics of actin and cell adhesion proteins during podosome disassembly and formation required for mobilization of myeloid cells during the immune response.
Funding: this work has been supported by grants from the Burton Myeloma Programme (YC and LM), the Wellcome Trust (JM, AJT, GEJ, and YC) and the Medical Research Council (GEJ), Great Ormond Street Children’s Charity (AJT).
The online version of this article has a Supplementary Appendix.
Authorship and Disclosures
The information provided by the authors about contributions from persons listed as authors and in acknowledgments is available with the full text of this paper at www.haematologica.org.
- Received June 1, 2011.
- Revision received November 11, 2011.
- Accepted November 15, 2011.
- Copyright© Ferrata Storti Foundation