- Marc Righini1⇑,
- Christelle Jobic2,
- Françoise Boehlen1,
- Jean Broussaud3,
- François Becker1,
- Morgan Jaffrelot4,
- Marc Blondon1,
- Bruno Guias5,
- Grégoire Le Gal2,
- the EDVIGE study group
- 1Division of Angiology and Hemostasis, Department of Medical Specialties, Geneva University Hospital and Faculty of Medicine, Geneva, Switzerland
- 2Université de Brest, INSERM CIC 05-02; CHU de la Cavale Blanche, Brest, France
- 3Department of Vascular Diseases, Centre Hospitalier Bretagne Atlantique, Vannes, France
- 4Emergency Department, CHU de la Cavale Blanche, Brest, France
- 5Vascular Medicine Physician, Morlaix, France
Contributors from the EDVIGE study group: Mohamed BABA-AHMED (Algiers), Dominique BERNARD (Carhaix), Henri BOUNAMEAUX (Genève), Luc BRESSOLLETTE (Brest), Paul CIRAFICI (Geneva), Cécile DE GAIL SAUTERON (Saint-Grégoire), Philippe DE MOERLOOSE (Genève), Aurélien DELLUC (Brest), Marie-Annick DIRER (Brest), Catherine DUIGOU-BIHi (Lorient), Pierre FONTANA (Geneva), Jacques GESTIN (Quimper), Salah GUEDDI (Geneva), Bruno GUICHAOUA (Quimper), Patrice HUDO (Saint-Brieuc), Nathalie JOURNEAUX (Rennes), Raymond KACZMAREK (Quimper), Geneviève KERCRET (Rennes), Marie-Luce LABALETTE (Lannion), Dominique LE BERRE (Paimpol), Isabelle LEBORGNE (Rennes), Marie-Pierre LE GOC PEDELUCQ (Lorient), Dominique LE MENN BUREAU (Pontivy), Fabrice LE THOER (Brest), Patrick LOUIS (Brest), Stéphanie LOUIS (Brest), Geneviève MADEC BOUGEARD (Lannion), Mario MAUFUS (Grenoble), Catherine NOEL MOREL (Pacé), Karine PICHON (Vannes), Emmanuel PLAT (Brest), Laurent PRUVOST (Saint-Malo), Philippe QUEHE (Brest), Edith RIVOAL (Quimper), Eric THULLIEZ (Rennes), Johny VOGEL (Sierre), Robert WUTSCHERT (Neuchatel).
The assessment of clinical probability represents an important step in the diagnostic strategy of patients with suspected deep vein thrombosis. The recently derived LEFt clinical prediction rule for pregnant women combines three variables: symptoms in the left leg (L), calf circumference difference of 2 centimeters or over (E for edema) and first trimester presentation (Ft) but is lacking an external validation. The LEFt rule was computed among pregnant women with suspected deep vein thrombosis who were included in a multicenter prospective diagnostic management outcome study. We calculated the proportion of women and the prevalence of deep vein thrombosis in each probability group, along with the diagnostic performances of the LEFt rule. All variables needed to compute the rule could be retrieved in 157 of the 167 pregnant women with suspected deep vein thrombosis. The prevalence of confirmed deep vein thrombosis was 13 of 157 (8.3%). The LEFt rule was negative in 46 (29%) women. A deep vein thrombosis was diagnosed in 13 of 111 (11.7%, 95% Confidence Interval (CI): 8.3-20.9%) of women with at least one of the LEFt criteria, as compared with none of 46 (0.0%, 95%CI: 0.0-7.9%) of women with none of the LEFt criteria. These results suggest that a negative LEFt rule accurately identifies pregnant women in whom the proportion of confirmed deep vein thrombosis appears to be very low. The rule should not be used as stand-alone test for excluding DVT during pregnancy, but might rather be implemented in a diagnostic strategy in association with D-dimer measurement and compression ultrasonography. The original trial was registered at clinicaltrials.gov (NCT 00740454).
During pregnancy, an accurate diagnosis is required in case of suspected deep vein thrombosis (DVT). Indeed, false positive tests lead to inappropriate anticoagulant treatment that increases the risk of bleeding and requires daily heparin injections during the entire pregnancy. Conversely, false negative tests might lead to a life-threatening thromboembolic event.
Clinical probability assessment by a clinical prediction rule (CPR) is a crucial step in the diagnostic management of a suspected DVT. However, the most commonly used CPR for DVT (the Wells' score)1 has never been validated in pregnant women. This rule is not suited to the pregnancy setting, since it includes items that are unlikely to be present in this younger and healthier population (e.g. age >65 years, cancer, recent surgery). Moreover, the diagnostic performance of clinical signs and symptoms is altered during pregnancy because pregnant women often experience symptoms compatible with DVT, and DVT symptoms may be different during pregnancy.2 On the other hand, some clinical findings, such as the left side presentation, may be more helpful during pregnancy. Finally, the proportion of confirmed DVT is lower in this setting than in other populations3,4 which may influence the performance of CPR.5
Recently, the LEFt clinical prediction rule was derived and internally validated by Chan et al. among 194 pregnant women investigated for a suspected DVT.6 This rule combines three variables: symptoms in the left leg (L), calf circumference difference of 2 centimeters or over (E for edema) and a first trimester presentation (Ft). They found no DVT among the 89 (46%) women with none of the LEFt criteria but in 7 of the 105 (16.2%) women with at least one LEFt criterion.
However, before the use of this clinical prediction rule may be recommended in clinical practice, external validation in an independent cohort is required. Thus, our aim was to externally validate the LEFt rule among pregnant women included in a European prospective diagnostic management outcome study.
Design and Methods
The study has been extensively reported elsewhere.7 Briefly, all consecutive pregnant or post-partum women referred for a suspicion of DVT to two tertiary care centers and 18 vascular medicine private practices between January 2006 and June 2009 were included in this study. Exclusion criteria included under 18 years of age, a suspicion of an associated PE, an ongoing anticoagulant treatment, an inability to give informed consent and an impossible follow up. The study was approved by the ethics committee of each institution.
Standardized report forms were filled in for all patients recording general characteristics, risk factors and clinical signs of VTE.
All women included in the study underwent a complete lower limb high-definition B-mode compression ultrasonography (CUS). DVT was ruled out in patients with a negative compression test and no visualized thrombus. DVT was diagnosed in cases of lack of compressibility of a deep vein and, for the iliac vein, in case of absence of Doppler flow or direct visualization of a thrombus.
All women with negative results of complete CUS were left without anticoagulant treatment and were followed-up for a three-month period. At the end of this follow up, all women were seen in the clinic or interviewed by phone by the study personnel using a standardized questionnaire to gather information about the 3-month period following the CUS. All suspected events were adjudicated by an independent committee that was blinded to the LEFt score.
Of the 210 women included in our diagnostic management study, the 43 postpartum women were excluded, leaving 167 available for analysis.
The LEFt score was computed post hoc on prospectively collected data. We estimated the association between the items of the CPR and the risk of DVT with a χ2 test or a Fisher's test, where applicable. We computed the LEFt score, and estimated the proportion of women in each clinical probability group, and the corresponding proportions of confirmed DVT, along with their 95% Confidence Intervals (95%CI). All analyses were performed using SPSS 19.0 software (IBM Inc., Somers, NY, USA).
Between January 2006 and July 2009, we consecutively included 167 pregnant women with suspected DVT. Data to compute the LEFt rule was missing for 10 women (6%) leaving 157 women available for this analysis. General characteristics of these 157 women are shown in Table 1. Mean age was 32 years (SD 6 years). There were 20 women included during the first, 46 included during the second, and 91 included during the third trimester of pregnancy. Overall, DVT was confirmed in 13 (8.3%) women during the initial evaluation, all of them involving proximal deep veins.
Table 2 shows study subject data according to items from the LEFt clinical prediction rule, along with the corresponding proportions of confirmed DVT. A suspicion in the left leg and the presence of edema were both significantly associated with the risk of DVT (OR 5.5, 95%CI: 1.2-25.7; OR 8.2, 95%CI: 2.4-28.4), while the association with the presentation during the first trimester (the third item of the LEFt rule) approached statistical significance (OR 3.6, 95%CI: 1.0-12.9) (Table 2).
The proportion of confirmed DVT increased with increasing LEFt rule scores (Table 3). Forty-six (29.3%) and 111 (70.7%) women were classified with an unlikely (no criteria) and likely (at least one criteria) probability of DVT, respectively (Table 3).
The repartition of women according to the number of criteria of the LEFt rule is shown in Table 3. The receiving operator characteristics (ROC) curve is shown in Figure 1. Area under the curve was 0.84 (95%CI: 0.73-0.94). In 46 women (29.3%), none of the criteria were present. No women in this group had a DVT either during the initial investigation or during follow up: none of 46, 0.0% (95%CI: 0.0-7.7%). The proportion of DVT was significantly higher in women with at least one criterion: 13 of 111, 11.7% (95%CI: 8.3-20.9). A negative LEFt rule had the following accuracy indices: sensitivity 100% (95%CI: 77-100%), specificity 32% (95%CI: 25-40%), negative predictive value 100% (95%CI: 92-100%), positive predictive value 12% (95%CI: 7-19%), negative likelihood ratio 0.0 (-) (Table 3).
In this study, we found that the LEFt rule accurately discriminates pregnant women with suspected DVT. Indeed, the proportion of DVT in patients with zero, one, two and three points was of none of 46 (0.0%), 4 of 83 (4.8%), 7 of 24 (29.2%) and 2 of 4 (50%), respectively. Area under the receiver operating characteristics (ROC) curves was 0.84 (95%CI: 0.73-0.94).
To our knowledge, this is the first external validation of the LEFt rule. We found similar diagnostic performances to those reported in the original paper: 100% sensitivity and negative predictive value. However, the proportion of patients with none of the LEFt criteria was somewhat lower in our study: 29% as compared with 46% in the study by Chan et al.6
To date, no formal clinical probability assessment tool has been available for suspected DVT during pregnancy. When assessing clinical probability, using a reproducible and accurate CPR is highly desirable. Indeed, empirical assessment of clinical probability may be associated with some limitations in pregnant women: infrequent manifestation, modified signs and symptoms, fear of venous thromboembolism (VTE) complications. In particular, the empirical clinical probability assessment is neither standardizable nor easily transmitted to less experienced clinicians. Moreover, the often-used Wells rule was not derived from nor has it been validated in pregnant women.
As compared with previously reported clinical prediction rules in VTE, the LEFt rule appears to perform very well: 1) The area under the ROC curve for the revised Geneva score for suspected PE and the Wells score for PE and DVT8,9 are usually around 0.7; 2) no clinical prediction rule to date has been able to identify a subgroup of patients with a null risk of confirmed VTE.5 Admittedly, the altered clinical presentation and lower threshold for suspicion in pregnant women accounts for the lower prevalence of DVT, which in turn improves the diagnostic performances of the rule.5 Pooling our results with those of Chan et al. none of 135 patients with a negative LEFt rule had DVT, corresponding to an upper limit of the 95% Confidence Interval of 2.8%. Of course, the rule should not be used as stand-alone test for excluding DVT during pregnancy. Further prospective studies need to be performed to validate this result.
How should the rule be used in everyday clinical practice? As previously stated, the rule should not be used as an exclusion tool. Indeed, even if none of the 46 women presenting without any of the LEFt criteria had a DVT during the 3-month formal follow up (none of 46, 0.0% (95%CI: 0.0-7.7%), the upper limit of the 95% Confidence Interval remains quite high and does not allow us to safely rule out DVT in this particular population. This is obviously in relation to the limited study sample. Identifying a subgroup at very low risk could be useful to simplify the diagnostic workup. For example, D-dimer levels increase during pregnancy and their usefulness is, therefore, reduced in this setting.9 The LEFt rule might be useful in combination with moderately sensitive D-dimer assays3 or using highly sensitive D-dimer tests with adapted threshold.10,11 Conversely, women with high LEFt score might require more extensive workup, such as serial compression ultrasound (CUS), other imaging modalities, or close clinical follow up. As a matter of fact, the 2 women in our study who experienced a thromboembolic event during the three months following a negative CUS had two points in the LEFt rule at initial presentation.
Of note, three more steps are missing before its implementation in daily clinical practice may be recommended. First, its diagnostic performance should be prospectively verified in an independent cohort of pregnant women. Second, its usefulness in a standardized diagnostic strategy should be assessed. For example, whether a higher threshold (e.g. low risk if < 2 LEFt criteria) could be used to increase the usefulness of the rule without altering its safety needs to be determined. Third, an impact study analysis should demonstrate that the use of the rule changes clinicians' behavior, improves outcomes and reduces costs.12
Some other findings deserve comment. First, we confirm the very large predominance of left leg involvement in pregnancy-related DVT: 11 of 13 (85%) of DVTs were left-sided. Also, all the diagnosed DVTs were proximal in our study whereas out of the context of pregnancy half of DVTs are limited to the calf.13
Our study has some limitations. First, the reference standard for DVT in our study was based on a single complete CUS.7 Although the 3-month thromboembolic risk in pregnant patients with a negative complete CUS was shown to be low enough to safely rule out DVT in a previous retrospective study,14 these results have not so far been reproduced by other investigators. Second, this is a post-hoc analysis. The rule was computed a posteriori after completion of the study. Third, our sample size was relatively limited, which produced wide confidence intervals around estimated proportions and diagnostic accuracy indices.
In conclusion, our study suggests that the LEFt rule accurately identifies pregnant patients at very low risk of DVT. Further studies need to be performed to clarify its role in the diagnostic management of pregnant women with suspected DVT.
We would like to thank all the residents and physicians from the emergency departments, radiology and ultrasonography units of all participating centers. We would also like to thank all study nurses, secretaries and clinical research associates for their invaluable help. And last but not least, we would like to express our gratitude to the patients who made the study possible by accepting to participate in the trial.
Funding: The study was supported by grants from the Projet Hospitalier de Recherche Clinique (Grant n. 2005 R 08.01) in France, and from the Swiss National Foundation (Grant n. 3200B0-120760) in Switzerland. The sponsors had no role in study design and the collection, analysis, and interpretation of data, or the writing of the article and the decision to submit it for publication.
Authorship and Disclosures: Information on authorship, contributions, and financial & other disclosures was provided by the authors and is available with the online version of this article at www.haematologica.org.
- Received June 12, 2012.
- Accepted September 19, 2012.
- Copyright© 2013 Ferrata Storti Foundation